【摘要】 目的检测激动素(kinetin)对大鼠肝纤维化后转化生长因子β1(transforming growth factor-β1,TGF-β1)和结缔组织生长因子(connective tissue growth factor,CTGF)含量变化的影响。方法将大鼠随机分为3组,模型组,用CCl4诱导形成肝纤维化模型;激动素组,CCl4造模同时给予0.1%激动素溶液0.5ml/100g/d(每天每100克体重大鼠注射0.5ml0.1%激动素溶液)皮下注射;对照组,给予生理盐水皮下注射,治疗12周。应用免疫组化和图像分析技术对3组中TGF-β1和CTGF含量及分布特点进行观察。结果激动素组TGF-β1为(1.339±0.244)%较模型组(1.904±0.367)%显著降低(P<0.01),CTGF为(2.689±0.534)%较模型组(4.242±1.612)%显著降低(P<0.01),上述两组TGF-β1和CTGF含量较正常对照组(0.926±0.277)%和(1.608±0.644)%显著升高(P<0.01)。结论激动素对实验性大鼠肝纤维化具有抑制作用。更多还原

【Abstract】 Objective To detect the effect of kinetin on the alteration of TGF-β1 and CTGF in CCl4-induced rat hepatic fibrosis after kinetin treatment.Methods The rats were divided into three groups:(1)model group,liver fibrosis was induced in rats by subcutaneous injection of CCl4;(2)kinetin group,at the time of making model,0.1% kinetin solution of 0.5ml/100g/d was injected subcutaneously;(3)control group,subcutaneous injection of normal saline lasted 12 weeks.The content and distribution of TGF-β1 and CTGF were observed with immunohistochemistry and image.Results The level of TGF-β1 in the kinetin treatment group was 1.339±0.244%,significantly lower than that in the model group 1.904±0.367%(P<0.01),and the level of CTGF in the treatment group was 2.689±0.534%,also significantly lower than that in the model group 4.242±1.612%(P<0.01).The TGF-β1 and CTGF levels of the two groups were significantly higher than those in the control group 0.926±0.277% and 1.608±0.644%(P<0.01).Conclusion Kinetin may have an inhibiting effect on CCl4-induced hepatic fibrosis in rats.更多还原