【摘要】 目的研究阿尔茨海默病(Alzheimer disease,AD)模型小鼠APP/PS1转基因小鼠脑内锌转运体ZNT7的分布和表达,探讨ZNT7参与Aβ老年斑形成的机理。方法应用免疫组织化学染色观察ZNT7在脑内分布情况,应用Western Blot方法分析ZNT7在APP/PS1转基因小鼠大脑内的表达。结果ZNT7免疫阳性反应产物主要分布在APP/PS1转基因小鼠大脑皮层、纹状体和海马的老年斑内,强阳性的ZNT7免疫产物定位于老年斑的核心。Western Blot分析结果表明ZNT7在APP/PS1转基因小鼠大脑内的表达明显高于野生型小鼠。结论ZNT7在APP/PS1转基因小鼠大脑内的高表达以及在Aβ老年斑的定位,提示ZNT7可能参与了锌离子在老年斑内的聚集,进而参与了APP/PS1转基因小鼠大脑内老年斑的形成。更多还原

【Abstract】 Objective To investigate the expression of the zinc transporter ZNT7 and its possible role in senile plaque formation in the brain of an animal model of Alzheimer’s disease,the APP/PS1 transgenic mouse.Methods Immunohistochemistry of ZNT7 was performed to observe the distribution of ZNT7 immunoreactivity,and Western blotting was used to analyze the expression of ZNT7 in the APP/PS1 transgenic mouse brain.Results ZNT7 immunoproducts were predominately distributed in the senile plaques in the neo-cortex,striatum and hippocampus of the APP/PS1 transgenic mouse brain.Strong ZNT7 immunoreactivity was found in the core of senile plaques.Western blotting results showed that high expression was measured in the APP/PS1 transgenic mouse brain.Conclusion Overexpression of ZNT7 and its localization in the plaqnes suggest that ZNT7 may be involved in the accumulation of zinc ions in the Aβ plaques of the APP/PS1 transgenic mouse brain.更多还原