【摘要】 在pH4.8的乙酸盐缓冲溶液中,邻苯二胺(OPD)形成粒径约380 nm的微粒,在392,420,445,484和507 nm处有5个较强的Rayleigh散射峰.辣根过氧化酶(HRP)催化H2O2氧化邻苯二胺生成黄色的2,3-二氨基吩嗪产物,反应体系在420,445和484 nm处的Rayleigh散射光信号显著减弱.在最佳条件下,HRP浓度在8.3×10-12~4.17×10-10g/mL范围内均与445和484 nm处的Rayleigh散射强度的降低值呈线性关系,其回归方程、相关系数、检出限(3σ)分别为ΔI445 nm=2.23c+11,ΔI484 nm=1.47c+4.8;0.9982,0.9919;3.6×10-12g/mL HRP和5.4×10-12g/mL HRP.该法用于辣根过氧化酶(HRP)的测定,结果满意.更多还原

【Abstract】 In pH 4.8 acetate buffer solution,o-phenylenediamine aggregated itself to particles in size of 380 nm,that exhibited five Rayleigh scattering peaks at 392,420,445,484 and 507 nm.Horseradish peroxidase(HRP) has a strong catalytic effect on the H2O2 oxidation of o-phenylenediamine to 2,3-diaminophenazine,that caused significantly decreasing of the Rayleigh scattering intensity at 420,445 and 484 nm.Under the optimal condition,the concentration of HRP in the range of 8.3×10-12-4.17×10-10 g/mL was all proportional to the decreased Rayleigh scattering intensity at 445 and 484 nm.Its regression equation,correlation coefficient and the detection limit were ΔI 445 nm=2.23c+11,ΔI484 nm=1.47c+4.8;0.9982,0.9919;3.6×10-12 g/mL and 5.4×10-12 g/mL HRP,respectively.A new enzyme catalytic-Rayleigh scattering spectral method was developed for assay of HRP,with a high sensitivity and selectivity,simplicity and rapidity.This assay was applied to the determination of HRP with satisfactory results.更多还原