【摘要】 通过对海带胁迫富硒培养,研究了海带对硒的富集总量及硒化学形态转变.建立了反相离子对缓冲盐等3个色谱系统的高效液相色谱与电感耦合等离子体质谱联用(RP-HPLC-ICP-MS)技术测定亚硒酸钠、硒甲基半胱氨酸(MeSeCys)、硒代蛋氨酸(SeMet)三种硒形态.采用3种提取溶剂,超声提取缩短提取时间,分离检测富硒海带的主要硒形态为亚硒酸钠、硒甲基半胱氨酸和硒代蛋氨酸.更多还原

【Abstract】 In the present study,the concentration and speciation of selenium in Laminaria,enriched with selenite during its growth,was performed by establishing a RP-HPLC-ICP-MS method to separate selenite,MeSeCys and SeMet successively.There were three of systems ion-pairing agent and buffer chromatograph:(1)Alltima C8 column,mobile phase:5 mmol/L citric,5 mmol/L hexanesulfonic acid(pH 3.5)-methonol(95∶5,volume ratio);(2)Alltima C8 column,mobile phase:water-methnol(98∶2,volume ratio)-0.1%TFA;(3)Alltima C18 column,mobile phase:20 mmol/L ammonium acetic-methonol(95∶5,volume ratio).Three solvent of 0.1 mol/L HCl,20 mmol/L Tris-HCl and 20 mmol/L ammonium acetic buffer were used for the extraction of seleno-compound from enriched Laminaria,the major selenospecies determined were selenite,MeSeCys,SeMet,compared with the relative retention time of Se standards.更多还原