【摘要】 研究利用PCR技术扩增出Erwinia herbicola的crtI基因,并连接到含强启动子Puc的表达载体pRKR5上构建表达质粒pRKR5-crtI,通过接合转移的方式将其导入光合细菌Rhodobacter sphaeroides突变株TC72中,调控氧浓度诱导工程菌累积红色色素,经HPLC和吸收光谱分析,工程菌中合成的色素为番茄红素,工程菌的生物量(干重)为2.36 g/L,番茄红素含量可达1.52 mg/g,与其他生产番茄红素的工程菌如大肠杆菌和产朊假丝酵母相比,番茄红素产量有一定程度的提高。更多还原

【Abstract】 In this research,the crtI of Erwinia herbicola was amplified by polymerase chain reaction and cloned into pRKR5 vector controlled by puc promoter from Rhodobacter sphaeroides,to construct pRKR5-crtI expression cassette.Then the expression construct was introduced into photosynthetic mutant bacteria TC72 by conjugative transfer.Induced by semiaerobic and aerobica conditions,the transformants could express crtI high efficiently and accumulate a red pigment quickly.The red pigment was testified as lycopene by absorbance spectra analysis and HPLC analysis.The engineering bacteria could grow up to 2.36 g/L and accumulate lycopene to 1.52 mg/g.Compared with other engineering bacteria producing lycopene,such as Escherichia coli and Candida utilis,the output of lycopene in the transformant had some increase.更多还原