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红玫瑰竹芋的离体培养与快速繁殖
In vitro culture and rapid propagation of Calathea roseopicta
【摘要】 以红玫瑰竹芋的地下分蘖芽为外植体进行了离体快繁研究,结果表明:芽启动诱导培养基以1/2MS+Adenine1.0 mg/L+6-BA 3.0 mg/L+NAA 0.05 mg/L为宜,诱导率达90.3%;增殖继代培养基以1/2MS+Adenine 1.0 mg/L+6-BA 2.0mg/L+NAA 0.50 mg/L为宜,每50 d继代1次,连续继代3次,增殖效果最佳,增殖系数达4.32倍;生根培养基以1/2MS+NAA 0.30 mg/L为宜,生根率达88.9%;将生根苗移栽于Klasmann泥炭422+Klasmann泥炭413(21)∶混合基质中,42 d后成活率达85.7%。
【Abstract】 Young shoots underground were used as explants to investigate optimum conditions for rapid propagation in Calathea roseopicta.The suitable shoot induction medium was 1/2MS+Adenine 1.0 mg/L+6-BA 3.0 mg/L+NAA 0.05 mg/L,the induction rate was 90.3%.The optimum medium for proliferation was 1/2MS+Adenine 1.0 mg/L+6-BA 2.0 mg/L+NAA 0.5 mg/L,in the medium the most efficient proliferation was obtained with 4.32 shoots per explant after three subcultures,one time per 50 days.The best medium for rooting was 1/2MS+NAA 0.3 mg/L,the rooting rate reached 88.9%.About 85.7% of plantlets survived after they were transferred onto the mixed substrates of Klasmann 422 and 413(2∶1) for 42 days.
- 【文献出处】 广东农业科学 ,Guangdong Agricultural Sciences , 编辑部邮箱 ,2008年03期
- 【分类号】S682.161
- 【被引频次】1
- 【下载频次】169