【摘要】 叶绿体遗传转化与传统的细胞核遗传转化相比具有许多独特的优点:外源基因表达效率高,可同时转化及表达多个基因,由于通过母性遗传,因此没有基因沉默现象及位置效应,也不会造成外源DNA的扩散。目前已经有超过四十多个外源基因整合到烟草等作物的叶绿体基因组中,表现出理想的农艺性状或作为生物反应器表达高水平的生物疫苗。本文对植物叶绿体基因组转化技术的原理和优点,外源基因导入叶绿体基因组的方法,以及目前叶绿体转化研究的最新进展进行了综述,并对其应用进行了展望。更多还原

【Abstract】 Chloroplast genetic engineering offers a number of unique advantages,including high-level transgene expression,multigene engineering in a single transformation event,transgene containment via maternal inheritance,lack of gene silencing,position and pleiotropic effects and undesirable foreign DNA.More than 40 transgenes have been stably integrated and expressed via the tobacco chloroplast genome to confer desired agronomic traits or express high levels of vaccine antigens and biopharmaceuticals.In this paper,the principles and advantages of the chloroplast genetic engineering,the methods used for chloroplast transformation,and the recent advances in the field were illustrated and the application of the technology in future was also discussed.更多还原