节点文献

转sbk和sck抗虫基因水稻的获得

Obtaining Transgenic Rice Plants Harboring sbk and sck Insecticidal Genes

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 张启军吕川根夏士健宗寿余漆庆明虞德容孙永华

【Author】 Zhang Qijun Lv Chuangen Xia Shijian Zong Shouyu Qi Qingming Yu Derong Sun Yonghua Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing Branch of National Rice Improvement Center, Nanjing, 210014

【机构】 江苏省农业科学院粮食作物研究所 国家水稻改良中心南京分中心江苏省农业科学院粮食作物研究所国家水稻改良中心南京分中心南京210014

【摘要】 转入多个抗虫基因是解决水稻抗虫的一条有效途径。本研究采用根癌农杆菌介导法将2个抗虫基因(sbk和sck)同时转入3个水稻品种中,通过一系列的分子检测(PCR和RT-PCR)分析,这2个基因已经成功转入3个水稻品种中并得到了表达;同时还获得1株不含抗生素筛选标记基因(hpt)和抗虫sbk基因,却含抗虫sck基因的转基因植株,命名为YJ27-5sck。用该植株作为供体,通过杂交将sck抗虫基因转育到另外5个水稻品种中且同样得到了表达,获得了不含筛选标记基因的转sck基因阳性植株。

【Abstract】 It might be one of the effective ways to solve the problems of rice insect harm by introducing multigenes into rice. In this research, two insecticidal genes (sbk and sck) have been transferred into three rice varieties mediated by Agrobacterium tumerfaciens approach, the results of PCR identifications indicated that the two genes have been integrated into the genomes of tested three rice varieties and the targeted genes would be successfully expressed. And also we distinguished a transgenic individual which its antibiotics select marker gene (hpt) and sbk insecticidal gene were negative but the sck insecticidal gene was positive, named as YJ27-5sck. By using the YJ27-5sck as insecticidal gene donor, we made some crosses with five other varieties, RT-PCR assay indicated that the sck gene might be introduced into their genomes and well expressed. This research might provide the transgenic germplasms for further transgenic breeding program.

【关键词】 水稻抗虫基因转基因植株基因表达
【Key words】 RiceInsecticidal geneTransgenic plantGene expressing
【基金】 国家高技术发展计划(2006AA100101);江苏省农业科学院基金(6110610)资助
  • 【文献出处】 分子植物育种 ,Molecular Plant Breeding , 编辑部邮箱 ,2008年01期
  • 【分类号】S511
  • 【被引频次】16
  • 【下载频次】260
节点文献中: 

本文链接的文献网络图示:

本文的引文网络