【摘要】 采用链霉亲和素-生物素-过氧化物酶复合物法(Strept actividin-biotin complex,SABC),观察低剂量X射线照射后小鼠睾丸各类生精细胞中(Apoptos isinducing factors,AIF)的表达变化;采用逆转录多聚酶链反应(Reverse transfer polymerase chain reaction,RT-PCR)法观察AIF的mRNA水平变化。研究低剂量X射线对小鼠睾丸生精细胞中凋亡诱导因子AIF蛋白及mRNA水平的影响。研究发现,0～0.2Gy照射后,小鼠睾丸各类生精细胞不同程度表达AIF,以精原细胞和精母细胞表达为主,精子细胞和精子则表达相对较少。AIF表达量与吸收剂量有相关性,0.075Gy照射组表达量最大,且发现AIF蛋白的表达随时间推移而增强,12h达到峰值,然后下降,但仍高于0h组。RT-PCR结果显示,低剂量照射12h后,0.1Gy剂量组AIF mRNA表达量最大。而0.075Gy照射后0～24h,其mRNA表达量逐渐增大,在24h到达峰值。所以,低剂量X线照射诱导小鼠睾丸生精细胞中AIF蛋白及mRNA表达与吸收剂量和表达时间有一定的相关性。更多还原

【Abstract】 In order to investigate the effects of low dose irradiation on the expression of apoptosis inducing fac-tors (AIF) protein and its mRNA in spermatogenic cells of mouse testis, immunohisto-chemical technique (SABC) and RT-PCR was used to observe the change of AIF protein and its mRNA expressions in the spermatogenic cells of the mouse testis irradiated with X-rays at different absorbed doses. After irradiation with 0～0.2Gy, the AIF protein expression occurred principally in spermatogonia and spermatocytes and less in spermatids and spermazoa. It was found that the AIF protein expressions increased with irradiation doses and reached the highest at absorbed dose of 0.075Gy, and then decreased gradually along with increasing doses. It was also found that the expression increased with the expression time and reached its top at 12h after irradiation, and then it decreased gradually but was higher than that of at 0h. In the RT-PCR, it was observed that the largest mRNA expressions occurred at 0.1Gy while irradia-tion doses changed from 0 to 0.2Gy and the peak of AIF mRNA expressions was at 24h after irradiation with 0.075Gy. As a conclusion, the expressions of AIF protein and its mRNA in spermatogenic cells of mouse testis induced by low-dose irradiation have some relationship with absorbed doses and expression time after irradiation with X-rays.更多还原