Rapid spectrophotometric quantification of meleumycin in colonies of streptomyces mycarofaciens via solid phase extraction in a sequential injection system
A rapid spectrophotometric method for the determination of meleumycin in colonies of streptomyces mycarofaciens was developed via solid-phase extraction in a sequential injection system.After meleumycin was released from the cell by ultrasonic lysis,2.0 mL of sample solution was directed to flow through a C18 packed mini-column.The meleumycin retained onto the column was afterwards eluted with 100 μL of methanol and the eluate was monitored at a wavelength of 232 nm.With a 2 mL sampling volume,an enrichment...
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