【摘要】 采用毛细管电泳柱后衍生激光诱导荧光测定3种卡那霉素类抗生素。研究了在反向电渗流条件下HAc-NaAc缓冲液酸度和浓度对卡那霉素类抗生素分离的影响,研究了Na2B4O7缓冲体系和柱后衍生试剂萘二醛/2-巯基乙醇浓度对检测信号的影响。采用50 mmol/L HAc-NaAc(pH5.0)+0.5 mmol/L CTMAB溶液为分离缓冲液,样品在-15 kV下分离,与柱后衍生试剂1.0 mmol/L NDA+8.0 mmol/L 2-ME+35 mmol/LNa2B4O7(pH10.0)的30%(V/V)甲醇溶液反应,激发诱导荧光检测卡那霉素类抗生素检出限为3.6×10-5~5.2×10-5g/L;本方法用于动物组织中卡那霉素类抗生素残留检测,相对标准偏差小于7.2%;回收率为90.2%~95.3%(n=4)。更多还原

【Abstract】 A selective and sensitive method for the determination of kanamycins with post-column derivatization in capillary zone electrophoresis and laser induced fluorescence detection was proposed.The derivatization reaction was carried out using a coaxial-gap reactor with a derivatization reagent of naphthalene-2,3-dicarboxaldehyde(NDA).The labeled kanamycins were detected with the excitation wavelength at 473 nm in laser induced fluorescence.The separation was carried out at-15 kV with reversed electroosmotic flow in 50 mmol/L sodium acetic buffer(pH 5.0) containing 0.5 mmol/L cetyltrimethyl ammonium.The derivatization was performed with the derivatization reagent of 1.0 mmol/L thalene-2,3-dicarboxaldehyde,8.0 mmol/L 2-merc-aptoethanol and 35 mmol/L soldium(pH 10.0) in 30%(V/V) methanol tetraborate buffer.The limits of detection for kanamycins ranging from 3.6×10-5 to 5.2×10-5 g/L and the separation efficiencies from 2.0×105 to 2.4×105 theoretical plates were obtained.The method has been employed to analyze kanamycin residues in animal tissues.更多还原