【摘要】 本研究探讨了在生理条件下,同时分离酸性和碱性蛋白质的毛细管电泳方法。在120mmol/L十二烷基硫酸钠(SDS)+5mmol/L丁二酸二异辛酯磺酸钠(AOT)+110mmol/L正辛烷+500mmol/L正丁醇+3mmol/LNa2B4O7+3mmol/LNaH2PO4(pH6.7)的微乳体系中,分离电压20kV,25min内完成核糖核酸A、细胞色素C、牛血清蛋白、α-淀粉酶和β-乳球蛋白5种不同酸、碱性蛋白质基线分离;分离效率为7.8×105～4.4×105/m;迁移时间的RSD为1.5%(n=11)。并讨论了蛋白质在AOT组成的复配微乳体系中的分离机制。更多还原

【Abstract】 A separation method of mixture of proteins by microemulsion electrokinetic chromatography(MEEKC)with surfactant sodium bis-(2-ethylhexyl)sulfosuccinate(AOT)was presented.The separation was carried out in a 50 cm(to the detector)50 m i.d.fused-silica capillary using a microemulsion system consisting of 110 mmol/L octane,120 mmol/L SDS,5 mmol/L AOT,500 mmol/L butanol in 3 mmol/L borate and 3 mmol/L NaH2PO4(pH 6.7)buffer.Under running voltage of 20 kV,the baseline resolution of the mixture of proteins was obtained in 25 min,the separation efficiency was in the range of 7.8×105-4.4×105/m,and the reproducibility of migration times was less than 1.5%(n=11).Optimum separation conditions were investigated.The electrophoretic behaviors of the proteins in the microemulsion systems with AOT were analyzed.It suggested that the system formed from SDS with AOT is a mixed microemulsion system with the reverse micelle(W/O)and normal micelle(O/W),and the migration of these proteins were governed by the enrichement in reverse micelle and the distribution in normal micelle mechanism.更多还原