【摘要】 采用Western blot方法检测进行热休克处理后恢复不同时间的V79细胞中热休克蛋白70(HSP70)的表达情况,采用克隆形成法观察经过热休克预处理的V79细胞经γ射线照射后细胞存活率的变化。实验结果显示:1)V79细胞经42℃热休克预处理恢复1小时后,热休克蛋白70即有明显增加,4小时开始达到高峰,可持续24小时;2)热休克预处理可以提高γ射线照射后V79细胞的存活分数(当照射剂量小于6 Gy时),说明热休克蛋白70对受辐射损伤的V79细胞具有保护作用。更多还原

【Abstract】 Western blot was used to detect the expression of heat shock protein 70 in V79 cells after heat shock pretreatment;V79 cells were irradiated using γ-ray after heat shock pretreatment,survival rate was observed using Colony formation assay.Our study shows that 1) the overexpression of heat shock protein 70 was observed in cells recovering for 1 hour after heat shock pretreatment,with peak expression in cells recovering for 4 hours,and could last for 24 hours;2) heat shock pretreatment was able to elevate survival rate of V79 cells after irradiation by 60Co γ ray(when the irradiation dose was less than 6 Gy).The results indicate that heat shock protein 70 has protective effect on radiation induced cell death of V79 cells(when the irradiation dose was less than 6 Gy).更多还原