【摘要】 利用RT-PCR、生物信息学及其启动区与GUS基因的融合表达分析等方法,分析了一个水稻CCCH型锌指基因OsZF77的表达模式。OsZF77开放阅读框包含843个核苷酸,编码280个氨基酸,含有2个CCCH锌指结构域。RT-PCR分析表明该基因在水稻种子的胚乳中具有较高丰度的表达而在胚中不表达。对OsZF77上游2.5 kb左右的启动子区段进行分析发现含有种子特异性表达必需的序列元件RY(CATGCATG)。进一步分析了该基因启动子区段与GUS基因融合表达载体的转基因水稻植株,结果表明GUS在种子胚乳特别是靠种皮的外围胚乳中具强表达,而在胚中检测不到GUS活性。以上结果说明该基因是一个胚乳优势表达基因。更多还原

【Abstract】 Using RT-PCR assay,bioinformatics prediction and expression of promoter-GUS fusion,the expression patterns of OsZF77 gene,a rice CCCH-type zinc finger gene,was studied.The OsZF77 ORF comprises 843 bp,and the predicted protein contains 280 amino acids with two CCCH domains.The RT-PCR analysis showed that OsZF77 gene was preferentially transcribed in rice endosperm and was not expressed in rice embryo and other vegetative organs.In a further analysis on the cis-elements of OsZF77 promoter region,a sequence component RY(CATGCATG),which was necessary for seed-specific expression,was found.In addition,the approximate 2.5 kb promoter region of OsZF77 gene was fused into GUS reporter gene and transformed into rice.The histochemical analysis revealed that GUS expression could not be detected in the transformed rice embryo,but clearly observed in the endosperm.These results indicated that OsZF77 gene might be an endosperm-preferential expressed gene.更多还原