【摘要】 目的研究喉癌耐药细胞系(LSC-1/TAX)中MDR1基因表达被沉默后肿瘤细胞对紫杉醇凋亡诱导作用的反应变化。方法表达MDR1 shRNA的慢病毒颗粒转染喉癌耐药细胞系LSC-1/TAX,观察干扰前后紫杉醇诱导喉癌细胞的凋亡改变。采用琼脂糖凝胶电泳进行凋亡定性实验;Giemsa染色观察凋亡细胞的形态学改变;Annexin V-APC/7-AAD双染后,流式细胞仪对凋亡细胞进行定量。结果药敏组细胞发生早期凋亡百分比为(50.95±4.47)%,实验组为(51.04±3.96)%,耐药组为(8.60±1.25)%,对照组为(9.05±1.57)%。实验组与耐药组差异有统计学意义(P<0.05)。喉癌耐药细胞系中MDR1表达被沉默后,细胞对紫杉醇的敏感性提高,凋亡增加。结论表达MDR1 shRNA的慢病毒颗粒可有效地干扰喉癌耐药细胞系中MDR1基因表达,提高喉癌细胞对紫杉醇的凋亡敏感性。更多还原

【Abstract】 OBJECTIVE To study the apoptosis changes induced by pactitaxel in human laryngeal cancer multidrug resistance cell line(LSC-1/TAX),which MDR1 gene expression was interfered.METHODS LSC-1/TAX was transfected by lentiviral particles that can express MDR1 shRNA.The apoptosis of cancer cells induced by paclitaxel was tested before and after the MDR1 gene was knocked down.To gain the purposes,three methods were employed:Agarose gel electrophoresis for qualitation, Giemsa stain for morphology observation,flow cytometry for quantitation.RESULTS The percent of early stage apoptosis in drug sensitive group,experimental group,drug resistant group,and control group by flow cytometry were(50.95±4.47)%,(51.04±3.96)%,(8.60±1.25)%,and(9.05±1.57)% respectively.Experimental group and drug resistant group had statistical difference (P<0.05).Once MDR1 gene expression had been silenced,the sensitivity of LSC-1/TAX cell to palitaxel was increased,and the cancer cells were induced to apoptosis.CONCLUSION Lentiviral particles expressing MDR1 shRNA can silence MDR1 gene effectively,thus the paclitaxel resistance phenotype of LSCI-1/TAX was reversed,and cell apoptosis to paclitaxel was increased.更多还原