【摘要】 目的:研究苦参碱(Mat)的体内抗肿瘤活性及机制.方法:采用动物移植性肿瘤实验法,腋窝皮下接种Lewis瘤株,随机分为模型组,环磷酰胺(CTX)组,单用Mat80,40,20mg/kg不同剂量组,CTX联合Mat组.灌胃给药21d后,计算平均抑瘤率,绘出肿瘤动态生长曲线,称小鼠体质量及各组肺、脾、肝、肾质量.应用放免法检测血清中表皮生长因子(EGF)含量.结果:单用Mat组对Lewis瘤株没有抑制作用(P>0.05).CTX联合Mat80mg/kg组,抑瘤率为56.78%,与CTX组抑瘤率42.69%相比较,抑瘤率升高,差异具有统计学意义(P<0.01).CTX联合Mat80,20mg/kg组血清中EGF浓度分别为(0.14±0.03),(0.11±0.02)mg/L,低于CTX组和模型组(P<0.01).结论:Mat可以增强CTX对小鼠Lewis肺癌的生长抑制作用,其可能机制与降低EGF有关.更多还原

【Abstract】 AIM:To study the antitumor activities and its mechanism of matrine (Mat) in vivo. METHODS:Lewis lung carcinoma (LLC) cells was transplanted into the subcutaneous space on the armpit of each C57BL mouse. Mice were randomized into tumor model,cyclophosphamide (CTX),Mat (80,40 and 20 mg/kg) alone and combination with CTX groups. After 21 d intragastric administration,the mean weight of the subcutaneous tumors was examined,average inhibition rate was analyzed,and then the average tumor growth curve was drawn. The body and viscera weight was examined. Radioimmunoassay (RIA) was used to determine the serum concentration of epidermal growth factor (EGF) in C57BL mice. RESULTS:The administration of Mat alone had no effect on LLC growth (P>0.05). The tumor control rate of Mat (80 mg/kg) combined with CTX group was 42.69%; compared with CTX group (56.78%),it increased significantly (P<0.01). In the group treated by Mat (80,20 mg/kg) and CTX,concentration of EGF in serum was (0.14±0.03) mg/L and (0.11±0.02) mg/L respectively,and it decreased significantly as compared with tumor model and CTX group (P<0.01). CONCLUSION:Mat can enhance the inhibition of CTX to the growth of mouse LLC,and its mechanism may be related with its action of decreasing the serum concentration of EGF.更多还原