【摘要】 目的:探讨毛脉酸模提取物二苯乙烯类成分与肝癌发生、发展的关系及其作用机制.方法:用二苯乙烯类成分处理人肝癌细胞株HepG248h后,提取药物处理组和对照组细胞的总RNA,将两组RNA纯化为mRNA,逆转录成cDNA,用Cy3和Cy5两种不同的荧光染料进行线性扩增标记,然后与人肿瘤相关基因表达谱芯片杂交,扫描后对获得的数据用LuxScan3.0软件分析.结果:经二苯乙烯类成分诱导后HepG2细胞的2474个肿瘤相关基因中,有GADD153,HPRG等5个基因表达明显上调,PRL-1,PAI-1等9个基因明显下调.结论:二苯乙烯类成分能改变细胞周期进程、细胞增殖、细胞凋亡、血管生成等相关基因的表达,此可能是其抗肿瘤作用的机制.更多还原

【Abstract】 AIM:To investigate the effects of chrysophenine from Rumex gmelini Turcz on the cancer-related gene expressions of HepG2 cells by gene expresion profile chip and to study its relationship with the development of hepatic cancer and the relative mechanisms.METHODS:The total RNAs were extracted from chrysophenine untreated or treated HepG2 cells to synthesize double-stranded cDNAs by reverse transcription.Then cDNA labeled with the incorporation of Cy3 and Cy5-dUT were amplified linearly.Probes were hybridized with oligonucleotide microarray.The chips were scanned and then analyzed by LuxScan 3.0 software.RESULTS:After chrysophenine induction,expressions of 9 out of 2474 cancer related genes,including PRL-1,PAI-1,etc,were down-regulated notably.Meanwhile,expressions of 5 out of 2474-cancer genes,including GADD153,HPRG,etc,were up-regulated markedly.CONCLUSION:The alterations in specific genes involved in modulating the cell cycle progress,cell proliferation,cell apoptosis,and antiangiogenesis may be responsible for anticancer molecular mechanism of chrysophenine.更多还原