【摘要】 目的:研究强磁重力环境(HMGE)对成骨细胞增殖、形态以及主要元素相对百分含量的影响.方法:采用大梯度超导磁体模拟空间重力环境,以细胞计数法和扫描电子显微镜结合能谱仪(SEM-EDS)分别检测强磁失重(0g),强磁常重力(1g)和强磁超重力(2g)三种HMGE对人骨肉瘤细胞(MG-63)及小鼠成骨样细胞(MC3T3)增殖能力、形态以及细胞中钙、钠、镁等元素百分含量的影响.结果:MG-63和MC3T3细胞在0,1,2g和对照环境中培养24h后,MC3T3细胞平均增殖倍数分别为:-(0.21±0.06),0.91±0.17,0.49±0.04和1.08±0.18;MG-63细胞平均增殖倍数分别为:1.03±0.16,0.75±0.06,0.67±0.15和0.44±0.07.与对照组相比较,0,1,2g环境处理组细胞形态发生明显变化,其中0g环境处理组变化最为明显.各环境处理组MC3T3细胞中钙、钠、镁元素百分含量与对照组相比差异不明显.MG-63细胞中钙元素百分含量0,1,2g环境处理组及对照组分别为(9.93±0.98)%,(3.61±1.53)%,(5.30±1.47)%和(7.35±1.92)%,与对照组相比,0g环境处理组MG-63细胞中钙元素含量明显增加(P<0.01),而1,2g环境处理组MG-63细胞中钙元素含量明显下降(P<0.05).各组MG-63细胞中钠元素含量与对照组相比较差异不明显.MG-63细胞中镁元素百分含量0,1,2g环境处理组及对照组分别为:0%,(1.90±0.77)%,(2.50±0.74)%和(2.83±0.21)%.0,1g环境处理组与对照组相比明显下降(P<0.05).结论:HMGE可影响成骨细胞的增殖、形态以及细胞中钠、镁、钙等元素的相对百分含量.更多还原

【Abstract】 AIM:To investigate the effects of high magneto-gravitational environment(HMGE)on the proliferation,morphology and the contents of main elements in osteoblasts.METHODS:A specially designed large gradient high magnetic field which could produce three apparent gravity levels(0,1 and 2 g)was used to simulate space gravity environment.The effects of HMGE on cell proliferation,morphology and the contents of main elements in osteoblasts were detected by cell counting assays,scanning electron microscopy and energy dispersive spectrometer after exposure to HMGE for 24 h.RESULTS:After being exposed to 0,1,2 g and control environment for 24 h,the average multiple of MC3T3 cell proliferation was-(0.21±0.06),0.91±0.17,0.49±0.04 and 1.08±0.18,respectively.The average multiple of MG-63 cell proliferation was 1.03±0.16,0.75±0.06,0.67±0.15 and 0.44±0.07,respectively.Compared to control environment,the morphology of MC3T3 and MG-63 cells exposed to 0,1 and 2 g environment was dramatically altered.HMGE(0,1 and 2 g)had no significant effects on the contents of sodium,magnesium and calcium in MC3T3 cells.The average percentage of calcium in MG-63 cells under 0,1,2 g and control environment was(9.93±0.98)%,(3.61±1.53)%,(5.30±1.47)% and(7.35±1.92)%,respectively.However,the differences of the average percentage of sodium in MG-63 cells between 0,1,2 g and control groups were not significant.The average percentage of magnesium in MG-63 cells under 0,1,2 g and control environment was 0%,(1.90±0.77)%,(2.50±0.74)% and(2.83±0.21)%,respectively.CONCLUSION:HMGE affects osteoblast proliferation,morphology and the relative contents of sodium,magnesium and calcium.更多还原