【摘要】 目的:探讨晚期糖基化终末产物(advanced glycation end products,AGE)及葡萄糖对THP-1巨噬细胞CXCL16 mRNA表达的影响.方法:①选择合适的培养时间;②不同浓度的AGE与THP-1巨噬细胞共同孵育;③不同浓度葡萄糖与THP-1巨噬细胞共同孵育;分别用AGE、葡萄糖及同时加入AGE和葡萄糖与THP-1巨噬细胞孵育;⑤采用RT-PCR检测孵育后CXCL16及CD36 mRNA的表达量,观察和比较各组孵育后CXCL16及CD36 mRNA的表达情况.结果:①随着培养液中AGE浓度的增加CXCL16及CD36 mRNA的表达增加;②随培养液中葡萄糖浓度的增加CXCL16及CD36 mRNA的表达增加;③AGE及葡萄糖同时作用于THP-1巨噬细胞时,对CXCL16及CD36 mRNA的表达上调作用大于AGE或葡萄糖单一因素的影响.结论:AGE及葡萄糖呈时间和浓度依赖性上调THP-1巨噬细胞CXCL16 mRNA的表达,并且AGE及葡萄糖两者合用可以进一步增加这种表达.这可能为糖尿病动脉粥样硬化机制的探讨提供新的线索.更多还原

【Abstract】 AIM:To investigate the effect of advanced glycation end products(AGE) and glucose on CXCL16 mRNA expression in THP-1 macrophages. METHODS: ①An appropriate incubation time was determined;②THP-1 macrophages were treated with AGE at different concentrations; ③THP-1 macrophages were treated with glucose at different concentrations; ④THP-1 macrophages were treated with AGE and glucose respectively and the mixture of AGE and glucose; ⑤CXCL16 and CD36 mRNA were determined by the reverse transcription-polymerase chain reaction(RT-PCR). The level of expression of CXCL16 and CD36 mRNA was compared within the different treatment groups. RESULTS: ①The expression of CXCL16 and CD36 mRNA was up-regulated with the concentration of AGE increased; ②The expression of CXCL16 and CD36 mRNA was also up-regulated with the concentration of glucose increasd; ③When THP-1 macrophages were treated with the mixture of AGE and glucose, the expression of CXCL16 and CD36 mRNA was higher than that of ones treated with AGE or glucose alone. CONCLUSION: The AGE and glucose can up-regulate the expression of CXCL16 mRNA in a time-dependent and dose-dependent manner in THP-1 macrophages,which may provide a new clue for the investigation of the mechanisms of diabetic atherosclerosis.更多还原