【摘要】 目的:通过检测不同维生素A(VA)状态对大鼠血清视黄醇水平与抗氧化能力的影响,分析两者之间的相关性,探讨体内重要的VA代谢中间产物视黄醇与机体脂质过氧化及抗氧化系统之间的关系.方法:9只健康雌性W istar大鼠随机分为正常对照组、VA缺乏组(VAD)和VA补充组(VAS),从交配前3 wk开始分别饲予VA缺乏饲料(含视黄醇400 U/kg)和正常饲料(含视黄醇6500 U/kg).正常对照组和VAD组仔鼠断乳后,继续饲予与其母鼠相同的饲料至生后8wk;VAS组仔鼠在出生0 d开始补充.高效液相技术检测血清视黄醇浓度,试剂盒检测血清抗氧化酶及产物超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及脂质过氧化物和丙二醛(MDA)水平.结果:VAD组仔鼠血清视黄醇浓度低于正常对照组和VAS组(P<0.05),VAS组血清视黄醇浓度与正常对照组无差异(P<0.05).与正常对照组相比,VAD组仔鼠血清抗氧化酶SOD和GSH-Px水平降低,而MDA水平升高,VAS组可观察到抗氧化酶SOD,GSH-Px和脂质过氧化物MDA水平恢复到正常对照组水平.血清中视黄醇水平与抗氧化酶SOD活性呈正相关(r=0.4574);血清中视黄醇水平与脂质过氧化产物MDA呈负相关(r=-0.4870);血清抗氧化酶GSH-Px活性与视黄醇水平无相关.结论:血清中视黄醇水平与抗氧化酶SOD活性、脂质过氧化物MDA水平密切相关,提示VA营养状态能够通过调节抗氧化酶活性和脂质过氧化反应而影响机体的抗氧化系统.更多还原

【Abstract】 AIM: To explore the effects of different vitamin A status on the serum retinol level and antioxidant abilities of rats and to discuss the correlation between serum retinol level and antioxidant abilities under different vitamin A status.METHODS: Nine Female Wistar rats(160-220 g) were used.From 3 weeks before coitus,3 rats were continually fed the standard diet,which comprised the basic diet supplemented with retinol(6500 U/kg).And other 6 rats received the vitamin A deficient diet,containing the basic diet and retinol(400 U/kg).Pups of the rats receiving the standard diet were fed the standard diet and designated as normal control group.The rats fed vitamin A deficient diet were randomly divided into vitamin A deficient(VAD) group and vitamin A supplement(VAS) group.The rats in VAD group received vitamin A deficient diet after birth and the rats in VAS group were fed the standard diet supplemented with retinol(50 000 U/kg·d) from birth for 7 days followed by standard diet.Each group comprised 8 pups selected randomly.All rats were executed at week 8 after birth.Serum retinol was collected and retinol level was monitored by HPLC.The total SOD activity,GSH-Px activity and MDA level were measured.RESULTS: The lower retinol level in serum was observed in the VAD group compared to the control and VAS group at week 8(P<0.05).But no significant difference in the cerebral cortex and hippocampus retinol level was observed between these 3 groups(P>0.05).The SOD and GSH-Px activity in serum was lower in AVD group than in control group,while the MDA level was higher,which both recovered by the vitamin A supplementation(P<0.05).The serum retinol concentration was positively correlated with the serum SOD activity(r=0.4574)and negatively correlated with the MDA level(r=-0.4870).No correlation between the GSH-Px activity and retinol level was observed.CONCLUSION: Serum retinol concentration has significant correlation with SOD activity and MDA level,which suggests that the vitamin A nutrition status can influence the antioxidant system through regulating the antioxidase activities and lipid peroxidation.更多还原