Breeding of Coenzyme Q₁₀ Produced Strain by Low-Energy Ion Implantation and Optimization of Coenzyme Q₁₀ Fermentation

【摘要】 <正> In order to increase the production efficiency of coenzyme Q₁₀,the original strain Agrobacterium tumefaciens ATCC 4452 was mutated by means of Nitrogen ions implantation. A mutant strain,ATX 12,with high contents of coenzyme Q₁₀was selected.Subsequently,the conditions such as carbohydrate concentration,nitrogen source concentration,inoculum’s size,seed age,aeration and temperature which might affect the production of CoQ₁₀were investigated in detail.Under optimal conditions,the maximum concentration of the intracellular CoQ₁₀reached 200.3 mg/L after 80 h fed-batch fermentation,about 245% increasing in CoQ₁₀production after ion implantation,compared to the original strain.更多还原

【Abstract】 In order to increase the production efficiency of coenzyme Q₁₀,the original strain Agrobacterium tumefaciens ATCC 4452 was mutated by means of Nitrogen ions implantation. A mutant strain,ATX 12,with high contents of coenzyme Q₁₀was selected.Subsequently,the conditions such as carbohydrate concentration,nitrogen source concentration,inoculum’s size,seed age,aeration and temperature which might affect the production of CoQ₁₀were investigated in detail.Under optimal conditions,the maximum concentration of the intracellular CoQ₁₀reached 200.3 mg/L after 80 h fed-batch fermentation,about 245% increasing in CoQ₁₀production after ion implantation,compared to the original strain.更多还原