Mutation Breeding of an Organic Phosphorus-Solubilizing Bacterium B3 by Low Energy Ion Beam Implantation

【摘要】 <正> A preliminary study on the mutation breeding of an organic phosphorus-solubilizingbacterium B3 by low-energy N⁺ ion beam was conducted.Analysis of dosage vs.survival rate andmutation rate showed that the dosage of 3×10¹⁵ions/cm² was optimal for the mutation.Someparameters for determining the efficient phosphorus content were studied to simplify the protocolsfor screening.Ultimately one reliable mutant was screened out under plating and flask-culturingscreening conditions.Importantly,a novel phosphorus-dissolving mechanism is reported for thefirst time in terms of bio-surfactant production,which indicates that bio-surfactant might play avital role in phosphorus-dissolving for some phosphobacteria.更多还原

【Abstract】 A preliminary study on the mutation breeding of an organic phosphorus-solubilizing bacterium B3 by low-energy N⁺ ion beam was conducted.Analysis of dosage vs.survival rate and mutation rate showed that the dosage of 3×10¹⁵ions/cm² was optimal for the mutation.Some parameters for determining the efficient phosphorus content were studied to simplify the protocols for screening.Ultimately one reliable mutant was screened out under plating and flask-culturing screening conditions.Importantly,a novel phosphorus-dissolving mechanism is reported for the first time in terms of bio-surfactant production,which indicates that bio-surfactant might play a vital role in phosphorus-dissolving for some phosphobacteria.更多还原