Mutation-Screening of Pleurotus Ferulae with High Temperature Tolerance by Nitrogen Ion Implantation

【摘要】 <正> In order to obtain Pleurotus ferulae with high temperature tolerance,conidiophoresof wild type strain ACK were implanted with nitrogen ions in energy of 5～15 keV and dose of1.5×10¹⁵～1.5×10¹⁶cm^-2and a mutant CGMCC1763 was isolated subsequently through ther-motolerant screening method.It was found that during riper period the surface layer mycelium ofthe mutant in mushroom bag wasn’t aging neither grew tegument even above 30℃.The myceliumendurable temperature of the mutant was increased by 5℃ compared to that of the wild typestrain.The fruiting bodies growth temperature of the mutant was 18～22℃ in daytime and8～14℃ at night.The highest growth temperature of fruiting bodies of the mutant was increasedabout 7℃ w.r.t,that of original strain.Through three generations investigations,it was foundthat the mutant CGMCC1763 was stable with high temperature tolerance.更多还原

【Abstract】 In order to obtain Pleurotus ferulae with high temperature tolerance,conidiophores of wild type strain ACK were implanted with nitrogen ions in energy of 5～15 keV and dose of 1.5×10¹⁵～1.5×10¹⁶cm^-2and a mutant CGMCC1763 was isolated subsequently through ther- motolerant screening method.It was found that during riper period the surface layer mycelium of the mutant in mushroom bag wasn’t aging neither grew tegument even above 30℃.The mycelium endurable temperature of the mutant was increased by 5℃ compared to that of the wild type strain.The fruiting bodies growth temperature of the mutant was 18～22℃ in daytime and 8～14℃ at night.The highest growth temperature of fruiting bodies of the mutant was increased about 7℃ w.r.t,that of original strain.Through three generations investigations,it was found that the mutant CGMCC1763 was stable with high temperature tolerance.更多还原