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基于rbcL和trnL-trnF序列探讨粤紫萁的系统位置及遗传关系
Phylogenetic Position and Genetic Relationship of Osmunda mildei(Osmundaceae):Evidence from rbcL Gene and trnL-trnF Region
【摘要】 通过对粤紫萁(Osmunda mildei C.Chr.)及紫萁属(Osmunda L.)植物的rbcL和trnL-trnF(包括trnL内含子和trnL-F间隔区)序列进行了测定,并结合部分已发表的紫萁科(Osmundaceae)植物的相关序列进行系统发育分析。结果表明:(1)所分析的紫萁属植物构成一个单系群;(2)粤紫萁与紫萁属中Sect.Plenasium组亲缘关系相近,聚成一支系,支持率为100%;(3)粤紫萁与Sect.Plenasium组的华南紫萁(O.vachellii Hook.)在碱基变异位点上高度一致,达94%,而与Sect.Osmunda组的紫萁(O.japonica Thunb)在变异位点上仅有2%的一致性。因此,根据叶绿体基因母系遗传的特性,推测华南紫萁为粤紫萁的母本,紫萁为其父本。
【Abstract】 In this paper,the rbcL gene and trnL-F region of chloroplast DNA from O.mildei and other Osmunda species were sequenced and the genetic relationships of O.mildei and other Osmunda species were studied.The results show that:(1)all Osmunda species studied in this paper form a monophyletic lineage;(2)O.mildei and Sect.Plenasium species form a clade;(3)O.mildei is highly cluster with O.vachellii in variable loci,approximately 94%,only 2% to the O.japonica.According to the plastid maternal inheritance law,our molecular evidences indicate that O.vachellii is the mother of hybrid species O.mildei and O.japonica is its father.
【Key words】 Osmunda mildei C.Chr.; chloroplast DNA sequences; phylogenetic position; genetic relationship;
- 【文献出处】 西北植物学报 ,Acta Botanica Boreali-Occidentalia Sinica , 编辑部邮箱 ,2008年11期
- 【分类号】Q941;Q943
- 【被引频次】16
- 【下载频次】293