【摘要】 目的:观察BMI-1(B-cell-specific moloney leukemia virus insert site 1)基因在白血病中的表达及其与白血病的关系。方法:应用逆转录-聚合酶链式反应(RT-PCR)技术,分别检测慢性粒细胞白血病细胞株(K562),26例慢性粒细胞白血病(CML)患者,6例慢性淋巴细胞白血病(CLL)患者,34例急性白血病(AL)患者和10例对照的骨髓单个核细胞(BMMNC)中BMI-1mRNA的表达情况,分析其在自血病中的表达规律及临床意义。结果:BMI-1基因在10例对照组标本中无表达;K562细胞株中BMI-1基因呈阳性表达;CML组中BMI-1基因的阳性率为15.4%,其中包括CML慢性期(CML-CP)及CML急变期(CML-AP),其阳性率分别为5%和50%;CLL组中BMI-1基因无表达;AL组中BMI-1基因表达率为47.1%,其中包括急性髓系自血病(AML)及急性淋巴细胞白血病(ALL),其阳性率分别为57.7%和12.9%;CML-CP组与CML-AP组阳性率比较差异有统计学意义(P<0.05),初诊AL中,AML组和ALL组阳性率比较差异有统计学意义(P<0.05),CML- AP组与初诊AL组阳性率比较差异无统计学意义(P>0.05)。对2例初诊BMI-1基因阳性表达患者随诊发现。初诊时BMI -1基因表达阳性,完全缓解后无表达,复发后再次表达,经再次治疗后仅达部分缓解,其BMI-1基因表达持续阳性。结论:BMI-1基因在白血病中存在较高的表达,在正常人及完全缓解病例中无表达,且与疾病的转归相关,提示BMI-1基因高表达可能参与白血病的发生、发展过程,有可能作为一种分子标志,为白血病的靶向治疗提供新的位点。更多还原

【Abstract】 Objective:To investigate the expression and its significance of BMI-1 geue in leukemia patients.Methods:BMI-1 gene level was assessed by reverse transcription polymerase ehain reaction(RT-PCR)in 34 acute leukemia patients’ bone marrow mononuclear cells(BMMNC),26 chronic myeloid leukemia(CML)patients,6 chronic lymphoblastic leukemia(CLL)patients,10 nor- mal controls,as well as leukemia cell line K562.The regularity of the expression of BMI-1 gene in leukemia and the relationship be- tween BMI-1 level and the prognosis of leukemia was explored.Results:The expression of BMI-1 gone was positive in leukemia cell line K562,but negative in 10 controls as well as CLL.The BMI-1 gone was expressed in 15.4%(n=4)CML patients and 47.1%(n =16)AL patients.The expression ratio of BMI-1 gone in CML-CR(5%)was significantly lower than that in CML-BP(50%)(P<0.05),and The expression ratio of BM1-1 gone between AML(57.7%)and ALL(12.9%)had statistical difference(P<0.05), while the expression ratio between CML-CR and AL has no statistical difference(P>0.05).The expression of BMI-1 gene positive in newly diagnosed leukemia changed to negative after complete remission,but the reverse transmition reoccurred after relapse.Conclu- sion:BMI-1 geue was overexpressed in leukemia patients,and may be related to curative effect of disease.BMI-1 gene expression may be involved in the occurrence and development process of leukemia,it may be used as a molecular marker for leukemia treatment targeting the prevision of new sites.更多还原