【摘要】 目的:检测H22肝癌荷瘤鼠体外活化脾淋巴细胞CD69分子的表达。方法:建立H22肝癌腹水瘤荷瘤鼠模型,电子天平称量小鼠体重,常规制备H22肝癌腹水瘤荷瘤鼠脾细胞悬液,ConA或H22肿瘤抗原体外活化,流式细胞仪检测CD69分子的表达。结果:荷瘤鼠的H22腹水瘤成瘤率为100%,平均体重为(32.03±3.44)g,高于生理盐水对照组的平均体重(19.15±1.14)g,两组差异有高度显著性(t=7.12,P=0.00)。荷瘤鼠体重变化随时间推移呈对数增长,而对照组呈平缓增长,两因素方差分析,荷瘤鼠与非荷瘤对照组之间差异有显著性(F=5.71,P=0.05),不同天数的体重差异有高度显著性(F=63.15,P=0.00)。荷瘤鼠脾淋巴细胞CD69的表达明显受到抑制,无论RPMI-1640对照组还是ConA活化组或H22肿瘤抗原活化组,荷瘤鼠CD69的表达率[(7.59±8.68),(19.42±12.57),(5.38±8.24)]均远低于非荷瘤对照组[(15.81±9.92),(44,96±13.84),(15.18±9.15)],经多因素方差分析,荷瘤鼠与非荷瘤鼠之间CD69表达率差异具有高度显著性(F=9.20,P=0.01),ConA活化与RPMI-1640对照之间CD69表达率差异亦有高度显著性(F=7.309,P=0.01)。但H22活化与RPMI-1640对照之间CD69表达差异没有显著性(F=1.09,P=0.31)。结论:H22肝癌荷瘤鼠淋巴细胞活化明显受到抑制。更多还原

【Abstract】 Objective:To detect the expression of CD69 molecular on activated spleen lymphocyte of H22 hepatoma bear- ing mice in vitro.Methods:The H22 hepatoma ascites baering mouse model was set up.The mice were weighed with electron- ic balance.The splenocyte suspension was prepared conventionally and activated with ConA or H22 tumor antigen in vitro.The expression of the CD69 molecular was detected with the flowcytometry.Results:The set up rate of the H22 hepatoma ascites bearing mice was 100% and the average weight of these mice was(32.03±3.44) g,which is higher than that of the NS control (19.15±1.14g) with the highly statistical significance (t=7.12,P=0.00).With the days going,the weight of the tumor bearing mice increased exponentially but the control increased gently instead.There were statistically significant difference be- tween tumor bearing mice and non-tumor control (F=5.71,P=0.05) and among the tumor bearing days (F=63.15,P= 0.00) acording to the multifactoral ANOVA.The expression of the CD69 molecular on the spleen lymphocyte of the tumor bearing mice was inhibited obviously and no matter the RPMI-1640 control or the ConA activating or the H22 tumor antigen ac- tivating,the expression rate of the CD69 on the spleen lymphocyte of the tumor bearing mice[(7.59±8.68 ),(19.42±12.57),(5.38±8.24) percent respectively] were obviously lower than those of non-tumor mice[(15.81±9.92),(44.96±13.84),(15.18±9.15) percent respectively].The difference was highly statistically significant between the tumor bearing and non tumor mice (F=9.20,P=0.01) and also highly statistically significant between ConA actvating and RPMI-1640 con- trol (F=7.309,P=0.01),but not statistically significant between the H22 tumor antigen activating and the RPMI-1640 con- trol (F=1.09,P=0.31).Conclusion:The activation of spleen lymphocyte in H22 hepatoma ascites bearing mice were inhib- ited obviously.更多还原