【摘要】 采用正向遗传学方法,从T-DNA标签技术构建的拟南芥突变体种子库中筛选到1株高叶绿素荧光表型的突变体(Hcf-1)。对该突变体及其子代群体进行Basta抗性及PCR分子检测,证实该突变体是由T-DNA插入引起的。突变性状与T-DNA共分离。对突变体(Hcf-1)和野生型(WT)叶片中色素含量、叶绿体内囊体膜色素蛋白复合物及蛋白亚基变化的分析结果表明,与野生型相比,突变体叶中的Chla和Chlb含量显著降低。光系统II部分解聚,其主要蛋白亚基CP43,CP47,D1,D2蛋白以及外周捕光色素天线蛋白LCHII含量均下降。光系统I基本未受到影响。据此判断由于T-DNA的插入,导致一个控制PSII发育的基因发生突变,叶绿体的发育和PSII的组装均受到较大的影响。更多还原

【Abstract】 According to forward genetics approaches,a photosynthesis mutant of high-chlorophyll-fluorescence phenotype has been isolated by screening the seeds of T-DNA Transformed Activation Tag lines.Test for Basta resistance to the mutant and its generations showed the mutants were all resistant,which indicated that the phenotype was co-segregated with resistance,and T-DNA insertion was further confirmed using PCR method.The pigments content and the components of chloroplast protein complexes were investigated by BN-PAGE and SDS-urea-PAGE.The results showed: the content of pigments decreased significantly in Hcf-1.The contents of PSII dimer,PSII monomer,CP43-less PSII and LHCII monomer decreased.The contents of some crucial small molecular proteins CP43,CP47,D1,D2 and LHCII monomer which are components of photosystem II were degraded,whereas the content of reaction centre protein psa A/B were little affected.From the results above we deduced that the mutant location may lie in PSII.更多还原