【摘要】 法呢基焦磷酸合酶(FPS)是治疗疟疾的特效药物——青蒿素生物合成途径的关键酶。运用RT-PCR方法从青蒿高产株系001中克隆法呢基焦磷酸合酶基因;构建His标签融合蛋白FPS表达载体,进行原核诱导表达、蛋白纯化及酶活性测定的研究。结果表明:克隆的FPS基因和文献已报道的2个青蒿FPS基因编码的氨基酸序列的同源性分别为98.83%和99.42%;原核诱导表达的His标签融合蛋白FPS的表达量高、可溶性好,具有FPS酶活性。更多还原

【Abstract】 Farnesyl diphosphate synthase(FPS)is a key enzyme in the biosynthetic pathway of arte- misinin,a new effective antimalarial drug.In this work,the fps was cloned from Artemisia annua strain 001 by RT-PCR; prokaryotic expression of FPS,purification,and enzyme activity assay were carried out.The results indicated that the homology of amino acid deduced from this FPS gene was 98.83% and 99.42% respectively to other two reported Artemisia annua fps; the His-tag FPS was expressed with high level,high solubility,and FPS enzyme activity.更多还原