【摘要】 目的:检测侵袭性牙周炎(aggressive periodontitis,AgP)患者龈下菌斑中的人巨细胞病毒(human cytome-galovirus,HCMV)、EB病毒1型(Epstein-Barr virus-1,EBV-1),探讨疱疹病毒与牙周致病菌及牙周临床指标间的关系。方法:应用巢式PCR技术,检测89名AgP患者和31名健康对照者龈下菌斑中的2种疱疹病毒:人巨细胞病毒(HCMV)、EB病毒1型(EBV-1),同时应用16S rRNA为基础的PCR技术检测8种牙周可疑致病菌,包括伴放线放线杆菌(Actinobacillus actinomycetemcomitans,Aa)、牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)、福赛类杆菌(Bacteroides forsythus,Bf;现已更名为Tannerella forsythensis,Tf)、中间普氏菌(Prevotella intermedia,Pi)、直形弯曲菌(Campylobacter rectus,Cr)、具核梭杆菌(Fusobacterium nucleatum,Fn)、牙密螺旋体(Treponema denticola,Td)、变黑普氏菌(Prevotella nigrescens,Pn)。结果:89名AgP患者的HCMV检出率为43.8%,显著高于健康对照者(12.9%),差异有统计学意义(P<0.01)。AgP组的EBV-1阳性率虽高于对照组,但差异无统计学意义(P>0.05)。检出6~8种牙周致病菌的AgP患者其HCMV和EBV-1检出率均明显高于仅检出3~5种细菌的AgP患者的病毒检出率(P<0.05)。结论:HCMV、EBV-1在AgP患者龈下的检出率较健康对照高;且在检出牙周致病菌种类数较多的样本中,疱疹病毒的检出率高,提示牙周感染HCMV和EBV-1与侵袭性牙周炎相关,疱疹病毒与牙周致病菌在侵袭性牙周炎的发病中可能起着协同作用。更多还原

【Abstract】 Objective:To evaluate the prevalence rates of human cytomegalovirus(HCMV) and Epstein-Barr virus-1(EBV-1) in subgingival plaque and analyze the relationship between herpesviruses,periodontal pathogenic bacteria and periodontal clinical parameters in Chinese patients with aggressive periodontitis(AgP).Methods: A total of one handred and twenty subgingival plaque samples were collec-ted from 89 AgP patients and 31 healthy subjects.HCMV and EBV-1 were detected using nested polymerase chain reaction(PCR).Contemporaneously,8 periodontal pathogenic bacteria including Actinobacillus actinomycetemcomitans(Aa),Porphyromonas gingivalis(Pg),Tannerella forsythensis(Tf),Prevotella intermedia(Pi),Campylobacter rectus(Cr),Fusobacterium nucleatum(Fn),Treponema denticola(Td),Prevotella nigrescens(Pn) were detected by 16S rRNA based PCR.Results: HCMV was more frequently detected in AgP patients(43.8%) than in healthy controls(12.9%,P<0.01).The prevalence rates of HCMV and EBV-1 in AgP patients with 6-8 kinds of bacteria detected were 54.4% and 17.4%,respectively,significantly higher than those with 3-5 kinds of bacteria detected(P<0.05).Conclusion: The prevalence rates of HCMV and EBV was higher in AgP patients than in healthy controls.Herpesviruses and periodontal pathogenic bacteria may cooperate synergistically in the development of AgP,which could be considered as a pathogenetic consortium in future investigation of periodontaltitis.更多还原