【摘要】 目的:比较胃黏膜不确定异型增生和异型增生组织p16基因甲基化状态,分析其与幽门螺杆菌(Helico-bacter pylori,Hp)感染等暴露因素的相关关系。方法:选取我国北方胃癌高发区(山东省临朐县)223例不确定性异型增生和130例异型增生胃黏膜,采用甲基化特异性聚合酶链反应和变性高效液相色谱技术,测定p16基因CpG岛甲基化状态,并与年龄、性别、吸烟、饮酒、Hp感染进行多因素分析。结果:胃黏膜不确定性异型增生和异型增生p16甲基化阳性率分别为28.3%和24.6%(P=0.46)。p16甲基化与年龄、性别、吸烟、饮酒均不存在相关关系(P>0.10)。单因素分析发现,在异型增生组织中p16甲基化与Hp感染接近统计学相关(P=0.07),Hp阳性者(93例)甲基化相对危险度高于阴性者(37例)(OR=2.62,95%CI∶0.92~7.43),多因素分析则二者无统计学关联(P=0.11)。结论:胃黏膜不确定性异型增生与异型增生p16甲基化频率相似;p16甲基化与年龄无相关关系:Hp感染可能与p16甲基化密切相关。更多还原

【Abstract】 Objective:To compare the CpG island methylation status of p16 in subjects with gastric indefinite dysplasia or dysplasia,and investigate its association with exposure factors.Methods: Methylation status of p16 was determined by methylation specific PCR method and denaturing high-performance liquid chromatography analysis in 223 indefinite dysplasia and 130 dysplasia from Linqu County in Shandong Province,an area with high GC mortality rates.Multivariable analysis was used to analyze the relationship between p16 methylation and age,gender,cigarette smoking,alcohol drinking,and Helicobacter pylori infection.Results: The methylation frequency of p16 in indefinite dysplasia was not significantly different from that of dysplasia(28.3% vs.24.6%,P=0.46).No significant association was found between p16 methylation and age,gender,cigarette smoking,and alcohol drinking.In dysplasia,the association between p16 methylation and Helicobacter pylori infection was close to the statistical significance by univariate analysis that the relative risk of p16 methylation of the infected subjects(n=93) is higher than that of the uninfected subjects(n=37)(OR=2.62,95%CI: 0.92 to 7.43,P=0.07).But by multivariate analysis,there was no association between them(P=0.11).Conclusion: The frequency of p16 methylation in gastric mucosa of indefinite dysplasia was similar to that of dysplasia.p16 methylation status was not associated with age,but might be significantly associated with Helicobacter pylori infection.更多还原