【摘要】 将反义Id4导入MEL细胞株,研究此基因对细胞增殖、分化及凋亡的影响.结果表明,Id4基因的沉默显著抑制细胞增殖,细胞的分化率明显提高(84倍);细胞的死亡率和凋亡率均增加,但基因沉默对分化的促进占优势,Id4可能是调控和保持分化的重要因子.反义Id4对细胞周期引擎基因、肿瘤抑制基因、凋亡抑制基因以及着丝粒蛋白基因表达的影响,说明Id基因的功能可以从细胞周期检验点调控等方面加以探讨.这些结果有利于细胞增殖、分化调节,特别是血细胞分化控制的研究.更多还原

【Abstract】 Antisense Id4 was transfected into MEL cells and effects on cell proliferation,differentiation and apoptosis were studied.Id4 knockdown suppressed cell proliferation,increased cell differentiation 84 times.Both necrosis and apoptosis were observed.Id4 may therefore be an important factor in differentiation maintenance also.Examination of antisense Id4 effects on cell cycle engine genes,tumor suppressor genes,apoptosis inhibitor genes and centromere protein genes revealed that Id may be involved in the regulation of cell cycle checkpoints.These data may have important implication for cell proliferation and differentiation,especially for the differentiation of blood cells.更多还原