【摘要】 检测散发性Alzheimer病(AD)患者与健康老年人血清雌二醇(E2)、睾酮(T)、促滤泡成熟素(FSH)、促黄体生成素(LH)水平,分析ApoE基因型和性激素水平与中国AD患者的相关性.采用放射免疫分析方法对81例Alzheimer病患者和98例正常老年人性激素水平测定,用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)方法分析ApoE基因型.结果为:AD组女性血清雌二醇37.25 pg.mL-1、男性睾酮2.22 ng.mL-1、促滤泡成熟素男性10.39 U.L-1、女性21.52 U.L-1、促黄体生成素男性7.54 U.L-1、女性9.42 U.L-1;正常组女性血清雌二醇39.09 pg.mL-1、男性睾酮4.07 ng.mL-1、促滤泡成熟素男性11.74 U.L-1、女性28.93 U.L-1、促黄体生成素男性1.62 U.L-1、女性6.28 U.L-1.AD组和正常对照组的ApoE基因频率存在差异,携带ApoE4的比例分别为16.1%、12.2%.ApoE4等位基因可能是中国散发性AD发生的危险因素.携带ApoE4基因型的AD患者血清雌二醇、睾酮水平明显低于对照组.提示雌二醇、睾酮水平下降可能与AD发病相关.更多还原

【Abstract】 To study apolipoprotein E gene polymorphism and sex hormone(E2、T、FSH、LH) level in Alzheimer’s patients.Eighty one patients with AD and 98 normal subjects were subject to ApoE allele genotyping by PCR-RFLP.E2,T,FSH(M/F),LH(M/F) was 37.25 pg·mL-1,2.22 ng·mL-1,10.39/21.52 U·L-1,7.54/9.42 U·L-1 and 39.09 pg·mL-1,4.07 ng·mL-1,11.74/28.93 U·L-1,1.62/6.28 U·L-1 in AD and normal subjects respectively.Frequency of ApoE4 in AD and normal subjects was 16.1% and 12.2% respectively.Work indicated that ApoEε4 allele gene is a risk factor for late-onset AD,AD patients with a ApoEε4 genotype had reduced levels of E2 and T compared with normal subjects.Data indicated that lowered level E2 and T predicts for a higher risk factor for AD.更多还原