【摘要】 从人心肌中提取心肌肌凝蛋白轻链(CMLC),免疫Balb/c小鼠,取其脾细胞与SP2/0骨髓瘤细胞做常规融合,制备单克隆抗体。通过抗人心肌肌凝蛋白轻链单克隆抗体的相加指数、亲和常数测定和抗体夹心实验,筛选到配对较好的两对单抗,在此基础上建立起轻链夹心ELISA方法,为临床急性心肌梗死早期诊断方法研究提供条件。更多还原

【Abstract】 Balb/c mice were immunized with cardiac myosin light chain-1(CMLC1)purified from human heart.The spleen lymphocytes were taken and fused with SP2/0 mouse myeloma cells to prepare the monoclonal antibody by a routine procedure.By measuring the additive indices,affinity constants and sandwich ELISA test,two pairs of monoclonal antibodies with better quality of combination were selected.The light chain sandwich ELISA method was set up for the diagnosis of acute myocardial infarction.更多还原