【摘要】 目的比较2种fimA基因型牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)刺激下口腔上皮细胞ICAM-1的表达水平。方法实验组用P. gingivalis ATCC 33277(I型菌毛组),W83(IV型菌毛组),47A-1(IV型菌毛组)分别与KB细胞(ATCC CCL17)共同孵育24h;对照组为未受P. gingivalis刺激的KB细胞。分别在1h、3h、6h和24h收集细胞,运用流式细胞仪检测KB细胞膜上ICAM-1的动态表达。结果P. gingivalis刺激细胞后3h、6h和24h,实验组ICAM-1的表达水平均高于对照组;2种fimA基因型P. gingivalis调节KB细胞表达ICAM-1的方式相似,IV型菌毛组的调节作用强于I型菌毛组。结论P. gingivalis上调口腔上皮细胞表达ICAM-1的水平与其fimA基因型相关,提示P. gingivalis致病性与其fimA基因型相关。更多还原

【Abstract】 Objective To investigate the intercellular adhesion molecule 1 (ICAM-1) expression in oral epithelial cells by challenge of P. gingivalis with different fimA genotypes. Methods P. gingivalis ATCC 33277 (type I),W83 (type IV) and 47A-1 (type IV) were assessed for their inductions of ICAM-1 expression in the human oral epithelial cells (KB cell line,ATCC CCL17). KB cells without the stimulation of P. gingivalis were used as control.ICAM-1 expression in KB cell membrane was determined by flow cytometry (FCM) at different time intervals (1 h,3 h,6 h and 24 h) following continuous co-culture of bacteria with KB cell line. Results Expression of ICAM-1 was up-regulated when KB cells were co-cultured with P. gingivalis at 3 h,6 h and 24 h. ICAM-1 expression way in oral epithelial cells by challenge of P. gingivalis with I fimA and IV fimA was similar,and strains with IV fimA were stronger than strains with I fimA. Conclusion fimA genotypes of P. gingivalis are related to the effect of ICAM-1 inductions,which indicates fimA genotype may be associated with pathogenesis of P. gingivalis.更多还原