【摘要】 [目的]观察川芎嗪(TMP)对体外培养内皮祖细胞(EPCs)功能影响和损伤保护作用,探讨它对冠心病的治疗作用。[方法]密度梯度离心法获取脐血单个核细胞,培养7天后,收集贴壁细胞,流式细胞仪、荧光显微镜法、matrigel试剂鉴定EPCs。收集贴壁细胞并分组为正常对照组,TMP(5mg/L、25mg/L、100mg/L、200mg/L)干预组,H2O2(500μmol/L)氧化应激模型组,TMP(5mg/L、25mg/L、100mg/L、200mg/L)和H2O2共同干预组,培养24h。分别观察EPCs增殖、粘附能力及凋亡率。[结果]与正常对照组相比,(5mg/L、25mg/L、100mg/L)TMP干预组对脐血来源的EPCs增殖、粘附功能无显著影响,(200mg/L)TMP干预组显著抑制脐血来源的EPCs增殖、粘附;与H2O2氧化应激损伤组相比,(5mg/L、25mg/L、100mg/L)TMP能显著降低H2O2对EPCs增殖、粘附抑制作用及细胞凋亡。[结论]低浓度TMP能保护EPCs氧化应激损伤,改善增殖、粘附能力,减少细胞凋亡,但对正常培养的EPCs增殖和粘粘功能无显著的促进或抑制作用。更多还原

【Abstract】 [Objective] This study was to investigate the effect of TMP on function of EPCs in normal or hydrogen peroxide(H2O2)inducing injury model cell culture,and to approach a therapy to coronary heart disease(CHD).[Method] Total mononuclear cells were isolated from the cord blood by ficoll density gradient centrifugation,cultured in vitro.After the cells cultured for 7 days,identified them by cellmarkers and the ability of intaking the ac-LDL,and made sure they were EPCs.To divide the cells into three groups,TMP(5,25,100,200mg),H2O2(500μΜ)model and TMP together with H2O2,cultured for 24h and then detected the function and apoptosis of EPCs.[Result] Versus to control group,the TMP(5,25,100mg)groups had no important effect on the proliferation and adhesion of EPCs,but TMP(200mg)group had negative effect on EPCs.Versus to H2O2 model group,TMP(5,25,100mg)preincubated groups had important protective effect on EPCs in proliferation,adhesion and apoptosis.[Conclusion] TMP could protect EPCs from oxidative stress injury,but had little benefit to EPCs in normal incubation.更多还原