节点文献

原位杂交检测人工感染鸭体内鸭瘟病毒的复制

Replication of Duck Plague Virus in Artificially Infected Ducks Detected by in situ Hybridization

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 程安春廖永洪朱德康汪铭书袁桂萍徐超韩晓英

【Author】 CHENG An-chun1,2,LIAO Yong-hong1,ZHU De-kang1,2,WANG Ming-shu1,2,YUAN Gui-ping1,XU Chao1,HAN Xiao-ying1(1.Avian Disease Research Center,College of Veterinary Medicine,Sichuan Agricultural University,Yaan 625014,China;2.Key Laboratory of Animal Disease and Human Health of Sichuan Province,Yaan 625014,China)

【机构】 四川农业大学动物医学院禽病防治研究中心四川农业大学动物医学院禽病防治研究中心 四川雅安625014 动物疫病与人类健康四川省重点实验室四川雅安625014四川雅安625014 动物疫病与人类健康四川省重点实验室

【Abstract】 Replication of duck plague virus(DPV) in artificially infected ducks were detected by in situ hybridization(ISH)which employed a 37bp oligonucleotide as probe designed according to DPV DNA sequence in GenBank.The results indicated that DPV DNA was detected in liver,intestine and bursa Fabricius at 4 h,in spleen and esophagus at 6h,in thymus at 12h post infection;DPV DNA in lung and kidney was detected only in dead ducks and no positive signal was detected in muscle,heart,cerebrum and pancreas.DPV DNA was distributed in cell nucleus and cytoplasm.Hepatocytes,sinus endodermal cells and Kuffer’s cells were the mainly infected cell types in liver.DPV DNA was mainly detected in epithelium of villi,in lamina propria of intestinal villi of duodenum,in stratum spinosum of esophagus,and in epithelium,cortex,medulla of bursa Fabricius.The positive signals were mainly detected in medulla of thymus,lymphocytes and macrophages of spleen.The research suggests that ISH is a direct and specific method in detecting DPV DNA in paraffin sections and it’s also a good method for virus diagnosis and DNA location of DPV.

【基金】 教育部“新世纪优秀人才支持计划项目(NCET-04-0906/NCET-06-0818);国家自然科学基金(30471297/30771598);四川杰出青年学科带头人基金后续资助项目(05ZQ026-038);四川省重点建设学科项目(SZD0418)
  • 【文献出处】 病毒学报 ,Chinese Journal of Virology , 编辑部邮箱 ,2008年01期
  • 【分类号】S858.32
  • 【被引频次】15
  • 【下载频次】173
节点文献中: 

本文链接的文献网络图示:

本文的引文网络