【摘要】 目的探讨Survivin和Caspase-3基因在非小细胞肺癌(NSCLC)中的表达及其相互关系。方法应用免疫组织化学等技术与方法检测74例NSCLC组织细胞中Survivin和Caspase-3基因的表达。结果在NSCLC组织细胞中,Survivin基因表达率为64.9%(48/74),其表达与NSCLC TNM分期、淋巴结转移和细胞分化程度密切相关(χ2=5.00~5.20,P<0.05);Caspase-3基因表达率为55.4%(41/74),其表达与淋巴结转移密切相关(χ2=5.98,P<0.05);在NSCLC组织细胞中,Survivin基因表达与Caspase-3基因表达呈高度负相关(r=-0.404,P<0.01)。结论在NSCLC组织细胞中,Survivin基因表达上调,Caspase-3基因失活或低表达;Survivin结合激活的Caspase-3,抑制其活性,阻止细胞凋亡;Survivin基因表达预示肺癌组织有较高的侵袭性和预后不良。更多还原

【Abstract】 Objective To investigate the expressions of Survivin and Caspase-3 gene and their relationship in non-small cell lung cancer(NSCLC). Methods The expressions of Survivin gene and caspase-3 gene in 74 paraffin-embedded NSCLC samples were detected immunohistochemically. Results Survivin was expressed in 48 of 74(64.9%) cases of NSCLC tissues,which was closely related to TNM staging,lymph node involvement,and cell differentiation(χ2=5.00-5.20,P<0.05).Caspase-3 was expressed in 41 of 74(55.4%) cases,which was closely related to lymph node involvement.The expression of Survivin was negatively correlated with Caspase-3 gene in NSCLC tissues(r=-0.404,P<0.01). Conclusion Over-expression of Survivin and non-expression of caspase-3 gene are seen in NSCLC.Over-expression of Survivin indicates higher invasiveness and poor prognosis of NSCLC by combining the active caspase-3 to inhibit the apoptosis.更多还原