节点文献

银莲花素A对S180、H22和U14细胞小鼠移植瘤的抑制作用

Antitumor Effects of Raddeanin A on S180,H22 and U14 Cell Xenografts in Mice

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 王明奎丁立生吴凤锷

【Author】 WANG Ming-Kui,DING Li-Sheng,WU Feng-E Natural Product Research Center,Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu,Sichuan,610041,P.R.China

【机构】 中国科学院成都生物研究所天然产物研究中心

【摘要】 背景与目的:有研究证明,从两头尖中分离得到的三萜皂甙银莲花素A在体外具有较好的抑瘤活性。本研究将观察银莲花素A在体内对小鼠移植瘤的抑制活性。方法:MTT法检测银莲花素A对人鼻咽癌KB细胞和人卵巢癌SKOV3细胞的抑制率。体内实验选取小鼠肉瘤S180细胞、小鼠肝癌H22细胞和小鼠宫颈癌U14细胞,观察银莲花素A注射给药对S180、H22和U14细胞小鼠移植瘤的抑瘤率,以及灌胃给药对小鼠肉瘤S180移植瘤的抑瘤率。测定银莲花素A的急性毒性。结果:银莲花素A在体外对KB细胞和SKOV3细胞的IC50分别为4.64!g/mL和1.40!g/mL。4.5mg/kg银莲花素A腹腔注射对S180、H22和U14细胞小鼠移植瘤的抑瘤率分别为60.5%、36.2%和61.8%。200mg/kg灌胃给药时抑瘤率为64.7%。灌胃给药和腹腔注射银莲花素A的LD50分别为1.1g/kg和16.1mg/kg。结论:银莲花素A在体内外均具有较好的抑瘤作用,体内可抑制小鼠移植瘤的生长,是一个有潜力的抗癌药物。

【Abstract】 BACKGROUND & OBJECTIVE:Raddeanin A,a triterpenoid saponin from Anemone raddeana Regel,has good antitumor activity in vitro.This study was to investigate its antitumor effects on tumor cell xenografts in mice.METHODS:The inhibitory effects of raddeanin A on the proliferation of human nasopharyngeal carcinoma KB cells and ovarian cancer SKOV3 cells were measured by MTT assay.The inhibitory effects of raddeanin A injection on the growth of sarcoma S180,liver cancer H22 and cervical carcinoma U14 cell xenografts in mice and the effect of raddeanin A lavage on the growth of S180 cell xenografts were measured.The acute toxicity of raddeanin A was also measured.RESULTS:The 50% inhibition concentration(IC50) of raddeanin A was 4.64 Ug/mL for KB cells and 1.40 Ug/mL for SKOV3 cells.When injected with raddeanin A at a dose of 4.5 mg/kg,the growth inhibition rates of S180,H22 and U14 cell xenografts were 60.5%,36.2% and 61.8%,respectively.When lavaged with raddeanin A at a dose of 200 mg/kg,the growth inhibition rate of S180 cell xenografts was 64.7%.The median lethal dose(LD50) of raddeanin A lavage was 1.1 g/kg and that of raddeanin A injection was 16.1 mg/kg.CONCLUSION:Raddeanin A has good antitumor activity both in vitro and in vivo,and would be a potential antitumor medicine.

  • 【文献出处】 癌症 ,Chinese Journal of Cancer , 编辑部邮箱 ,2008年09期
  • 【分类号】R73-36
  • 【被引频次】34
  • 【下载频次】413
节点文献中: 

本文链接的文献网络图示:

本文的引文网络