【摘要】 背景与目的:个体遗传易感性对胃癌的发生发展具有重要作用,其中免疫抑制因子白细胞介素10(interleukin-10,IL-10)基因-1082G/A位点单核苷酸多态性(single anucleotide polymorphism,SNP)引起研究者重视。本研究分析IL-10-1082G/A SNP在中国北方胃癌高发区和低发区人群中的分布,探讨IL-10-1082G/A SNP与胃癌发病风险的关系。方法:1516例研究对象来自胃癌高发区辽宁庄河(983例)及低发区沈阳(533例),采用聚合酶链反应-限制性片段长度多态(polymerase chain reaction-restriction fragment lengthpolymorphism,PCR-RFLP)方法检测该人群中IL-10-1082G/A位点单核苷酸多态性;采用酶联免疫吸附实验(enzyme-linked immunosorbent assay,ELISA)检测血清幽门螺杆菌(Helicobacter pylori,H.pylori)IgG。采用病理组织学诊断进行疾病分组,按性别和年龄配对,选取基本正常、浅表性胃炎、胃糜烂溃疡、萎缩性胃炎和胃癌组织各111例,用于IL-10-1082G/A SNP与胃癌发病风险的分析。结果:中国北方人群IL-10-1082G/A基因位点AA、AG、GG三种基因型分布频率分别为88.5%、10.9%、0.6%。IL-10-1082AG+GG基因型在胃癌组、非胃癌组及正常对照组分布频率分别为19.8%、9.7%和6.3%,IL-10-1082AG+GG基因型在胃癌高、低发区人群中的分布的地区及性别差异无统计学意义(P>0.05),而胃癌组高于非胃癌组(P=0.003)及正常对照组(P=0.003),其差异均有统计学意义。以IL-10-1082AA基因型并H.pylori IgG阴性的正常组为对照,IL-10-1082AG+GG基因型并H.pylori IgG抗体阴性个体、IL-10-1082AA基因型或AG+GG基因型并H.pylori IgG抗体阳性个体胃癌患病风险均提高,OR(95%CI)分别为3.3(1.3～8.6)、4.3(2.0～9.5)、2.5(2.1～3.1),但三组两两进行比较其差异均无统计学意义(P>0.05)。结论:携带IL-10-1082AG+GG基因型个体胃癌的发病风险提高,IL-10-1082G/A SNP和H.pylori感染在胃癌发生发展过程中无交互作用。更多还原

【Abstract】 BACKGROUND & OBJECTIVE: The individual genetic susceptibility is important in the development of gastric cancer. The 1082G/A single nucleotide polymorphism (SNP) of interleukin-10 (IL-10), an immune suppressor gene, became a research hot spot in this field. This study was to analyze the distribution of IL-10-1082G/A SNP in a population from northern China, and explore its correlation to the susceptibility to gastric cancer. METHODS: Blood samples were taken from 983 subjects in a high risk area of gastric cancer and 533 in a low risk area. The genotype of IL-10-1082G/A was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Serum level of anti-Helicobacter pylori (H. pylori) IgG was measured by enzyme-linked immunosorbent assay. Matched by sex and age, 111 specimens of normal gastric mucosa (NOR), 111 specimens of superficial gastritis (GS), 111 specimens of gastric erosion ulcer (GEU), 111 specimens of atrophic gastritis (AG) and 111 specimens of gastric cancer (GC) were selected to analyze the correlation of IL-10-1082G/A SNP to the susceptibility to gastric cancer. RESULTS: The detection rates of IL-10-1082 AA, AG, GG genotypes in the 1516 subjects were 88.5%, 10.9% and 0.6%, respectively. There were no differences in region and sex distribution of IL-10-1082AG+GG genotype between the high and low risk areas of gastric cancer. The detection rate of IL-10-1082 AG+GG genotype was significantly higher in gastric cancer than in benign lesions and normal mucosa (19.8% vs. 9.7% and 6.3%, P=0.003). As compared with the subjects with IL-10-1082 AA genotype and without H.pylori infection, the subjects with AG+GG genotype and without H.pylori infection odds ratio (OR)=3.3, 95% confidence interval (CI)=1.3-8.6, the subjects with AA genotype and H.pylori infection (OR=4.3, 95% CI=2.0-9.5) and the subjects with AG+GG genotype and H. pylori infection OR=2.5, 95% CI=2.1-3.1 had higher susceptibility to gastric cancer, but there was no significant difference between every 2 of the 3 groups (P>0.05). CONCLUSIONS: The susceptibility to gastric cancer is higher in the subjects with IL-10-1082 AG+GG genotype. There is no synergism between IL-10-1082G/A SNP and H.pylori infection.更多还原