【摘要】 目的研究穿心莲内酯对非酒精性脂肪性肝炎大鼠(Nonalcoholic steatohepatitis,NASH)的防治作用。方法SD大鼠40只,雌雄各半,随机分为对照组、模型组、异甘草酸镁组(Magnesium isoglycyrrhizinate,MgIG)(10 mg.kg-1)及穿心莲内酯组(an-drographolide,AGH)(100 mg.kg-1)。除对照组给予生理盐水外,其余各组每天上午给予脂肪乳剂灌胃;每天下午除给生理盐水外,其它组分别腹腔注射相应药物。3周后处死动物,测定血清甘油三酯(TG)、总胆固醇(TC)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、丙二醛(MDA)、超氧化物歧化酶(SOD)、肿瘤坏死因子-α(TNF-α)和肝匀浆TG、TC、MDA、SOD,并行肝脏病理学检查。结果模型组大鼠出现NASH,MgIG组及AGH组血清和肝脏TG、MDA、TNF-α均显著降低,肝组织SOD活性明显降低;肝脂肪变性和炎症程度明显减轻。结论穿心莲内酯对脂肪乳剂诱导的NASH大鼠具有防治作用。更多还原

【Abstract】 Aim To study preventive effects of andrographolide on NASH.Methods 40SD rats were randomized into control,model,magnesium isoglycyrrhizinat(10 mg·kg-1)and andrographolide(100 mg·kg1)group(n=10).The rats of the other groups were fed with a standard diet and fat emulsion(10 ml·kg-1),while the rats of control group were only fed with a standard diet.At the same time,the control and model groups received intraperitoneal injection with NS,while the other groups received intraperitoneal injection and magnesium isoglycyrrhizinat or andrographolide.The serum TG,TC,ALT,AST,MDA,SOD,TNF-α and hepatic TG,TC,MDA,SOD were detected histologically.Results The model group developed NASH after 3 weeks.MgIG and andrographolide(100 mg·kg-1)significantly reduced TG TC,MDA,SOD of the serum and liver tissue,and elevated SOD in liver tissue,and also ameliorated hepatocellular macrovesicular steatosis.Conclusions Andrographolide has protective and therapeutic effects on high fat diet induced NASH in rats.更多还原