【摘要】 目的分析羊水细胞染色体核型,探讨其在产前诊断中的应用。方法抽取104例孕16～29周孕妇的羊水,采用改良的羊水细胞培养方法进行培养。结果103例一次培养成功,1例培养失败后复穿培养成功。103例中98例原瓶培养即获得足够染色体分裂相,5例由于细胞克隆数少而依靠传代培养获得结果。共检出异态性4例,异常核型4例,其中21三体1例,结构异常3例。结论羊水细胞培养染色体检查进行产前诊断安全可靠。更多还原

【Abstract】 Objective To analyze the karyotyping of amniotic cells and explore its application in prenatal diagnosis.Methods 104 samples of amniotic fluid from pregnant women during 16-29th-week gestation were cultured and karyotyped.Results 103 cases were successfully cultured,among which 98 cases were successful at first time,and 5 cases were later cultured for more cells due to the less number of colone.Finally 4 cases of chromosomal abnormality were identified,of which,1 case was trisomy and the others were heteromorphism.Conclusion It is safe and practicable to culture amniotic fluid cells and analyze the karyotyping in prenatal diagnosis.更多还原