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放线菌Lj20发酵液杀虫活性的研究

Study on the Insecticidal Activity of Fermentation Liquid of Actinomycete Lj20

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【作者】 范永玲; 史赟; 刘秀英; 张喜娃; 马林; 韩巨才;

【Author】 FAN Yong-ling et al(Shanxi Meteorological Bureau,Taiyuan,Shanxi 030002)

【机构】 山西省气象局; 山西农业大学农学院; 山西省气象科技服务中心; 山西农业大学农学院 山西太原030002; 山西太谷030801; 山西太原030002;

【摘要】 [目的]为抗生素类药剂的开发和合理使用提供参考。[方法]从辣椒根部分离得到植物内生放线菌Lj20,研究其发酵液对小菜蛾幼虫、朱砂叶螨成螨及产卵的杀虫活性。[结果]处理24 h后,植物内生放线菌Lj20的发酵原液对小菜蛾幼虫的选择性拒食率、非选择性拒食率分别为95.45%9、7.84%,处理48 h后分别为81.55%、96.51%;对朱砂叶螨成螨的校正死亡率最高,为54.51%,随着稀释倍数的增加,校正死亡率逐渐降低。发酵原液和不同稀释倍数的发酵液对朱砂叶螨都具有较强的产卵忌避活性,处理24 h后原液的产卵忌避率高达90.24%。[结论]植物内生放线菌Lj20的发酵液对小菜蛾幼虫有较强的拒食作用,对朱砂叶螨有较强的触杀作用和产卵忌避作用。

【Abstract】 [Objective] The aim was to provide reference for the development and reasonable usage of antibiotics reagent.[Method] Endophytic actinomycete Lj20 was separated from pepper root.The insecticidal activities of its fermentation liquid on larvae of Plutella xylostella,adults and eggs of Tetranychus cinnabarinus were studied.[Result] After treatment for 24 h,the selective and non selective antifeedant rates of the crude fermentation liquid of Lj20 on P.xylostella larvae were 95.45% and 97.84% resp.and after treatment for 48 h,they were 81.55% and 96.51% resp.The corrected mortality on T.cinnabarinus adult was highest,being 54.51% and decreased gradually along with the increase of dilution times.Both the crude fermentation liquid and the fermentation broth diluted at different times had stronger oviposition deterring activity on T.cinnabarinus.After treatment for 24 h,the oviposition deterring rate of crude fermentation broth was up to 90.24%.[Conclusion] The fermentation broth of endophytic actinomycete Lj20 had stronger antifeedant effect on P.xylostella larvae and stronger contact and oviposition deterring effect on T.cinnabarinus.

【基金】 山西省留学基金(200441);山西省科技攻关项目(051045)资助
  • 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2008年14期
  • 【分类号】S482.3
  • 【被引频次】10
  • 【下载频次】136
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