【摘要】 [目的]研究蓝莓组培繁育技术,为发展蓝莓产业化生产提供技术支撑。[方法]以6个兔眼蓝莓品种、5个高丛蓝莓品种和2个半高丛蓝莓品种的带芽茎段为外植体,进行丛生芽的诱导培养、继代培养、生根和温室锻炼和大田移栽试验,还进行了蓝莓扦插繁育技术试验。[结果]不同的蓝莓具有不同的最佳繁育方法,高丛和半高丛蓝莓适宜组培繁育,而兔眼蓝莓适宜绿枝扦插繁育。蓝莓在丛生芽诱导培养基WPM+ZT 2.0 mg/L、继代增殖培养基WPM+ZT1.0 mg/L、生根培养基1/2WPM+IBA 0.1 mg/L培养,生根率达30%～70%,试管苗经温室锻炼后成活率达60%。[结论]安徽发展蓝莓产业化生产条件优越,具有广阔前景。更多还原

【Abstract】 [Objective] The research aimed to study the technologies of tissue culture and breeding in blueberry and provide the technical support for developing the industrial production of blueberry.[Method] Taking the stem with buds in 6 Vaccinium ashei varieties,5 high bush blueberry varieties and 2 half-high bush blueberry varieties as explants,the induction culture,subculture,rooting,greenhouse training and field transplanting tests were made for the clustered buds.And the cutting breeding test of blueberry was also made.[Result] Different blueberry variety had different best breeding method.High bush blueberry varieties and half-high bush blueberry varieties were suitable for the tissue culture breeding,while V.ashei varieties were suitable for cutting breeding with green branches.When blueberry was cultured on the clustered bud induction medium WPM +ZT 2.0 mg/L,the subculture medium WPM + ZT1.0 mg/L and the rooting medium 1/ 2WPM + IBA 0.1 mg/L,the rooting rate reached 30 %～70 % and the survival rate of the test-tube seedlings after greenhouse training reached 60 %.[Conclusion] The development of the industrialized production of blueberry in Anhui province had predominant conditions and broad foreground.更多还原