【摘要】 [目的]为研究花生居群遗传多样性奠定基础。[方法]以汕油162和sunolicn 95R为模板对花生进行SRAP扩增,研究模板DNA浓度、Mg2+浓度、引物浓度和TaqDNA聚合酶的用量对SRAP扩增效果的影响,探索花生SRAP反应的最佳条件。[结果]在一定范围内,模板DNA含量和TaqDNA聚合酶的用量对花生SRAP扩增结果的影响较小。当Mg2+浓度为2.5 mmol/L时,扩增效果最佳。当Mg2+浓度逐渐降低时,扩增条带逐渐变弱。引物浓度为0.5 mmol/L时能够扩增出清晰、重复性好的条带。花生SRAP反应的最佳条件为:模板DNA含量为100 ng,Mg2+浓度为2.5 mmol/L,引物浓度为0.5 mmol/L,TaqDNA聚合酶的用量为1 U。[结论]与AFL P技术相比,该研究中所建立的花生SRAP反应体系更为简便和可靠。更多还原

【Abstract】 [Objective] The aim of the research was to lay the foundation for studying the genetic diversity of Arachis hypogaea L.populations.[Method] With Shanyou 162,sunoliec 95R as template,SRAP amplification was made on A.hypogaea to study the effects of the concn.of template DNA,Mg2+ concn.,primer concn.and the dosage of Taq DNA polymerase on the effect of SRAP amplification.And the optimum conditions for SRAP reaction of A.hypogaea were discussed.[Result] In a certain range,template DNA content and the dosage of Taq DNA polymerase had little effects on the results of SRAP amplification for A.hypogaea.When Mg2+ was 2.5 mmol/L,the amplification results were best.When Mg2+ concn.was gradually decreased,the amplified bands were gradually weakened.When the primer was 0.5 mmol/L,the clear bands with good repeatability could be amplified.The optimum conditions for SRAP reaction of A.hypogaea were as follows: template DNA content was 100 ng,Mg2+ was 2.5 mmol/L,primer was 0.5 mmol/L and the dosage of Taq DNA polymerase was 1 U.[Conclusion] Compared with AFL P technology,the SRAP reaction system for A.hypogaea established in this research was more convenient and reliable.更多还原