【摘要】 [目的]为建立一种检测饲料中硝基咪唑类药物的HPLC方法。[方法]提取饲料样品中的甲硝唑、二甲硝唑、罗硝唑和替硝唑,用Oasis MCX小柱进行净化后,以甲醇-水溶液为流动相,梯度洗脱,紫外检测波长为320 nm,进行HPLC测定。[结果]甲硝唑、罗硝唑、二甲硝唑和替硝唑的检测限分别为15.7、25.3、27.3和54.9μg/kg。在0.1～10.0μg/ml浓度范围内,4种硝基咪唑类药物色谱峰面积与浓度呈线性相关,相关系数为0.993～0.999,符合对饲料中抗生素分析的要求。4种硝基咪唑类药物平均回收率为82.5%～99.7%,符合对饲料中药物添加剂分析的准确度要求,变异系数为3.5%～14.0%,符合残留分析对精密度要求。[结论]该方法操作简单、结果准确,可用于测定饲料中硝基咪唑类药物的含量。更多还原

【Abstract】 [Objective] The research aimed to establish a kind of HPLC method for detecting nitroimidazole drugs in feeds.[Method] Metronidazole,dimetridazole,ronidazole and tinidazole were extracted from feed samples.They were purified by using Oasis MCX columella and determined with HPLC at the ultraviolet detection wavelength of 320 nm by taking methanol-water solution as mobile phase and using the technology of gradient elution.[Result] The detection limit for metronidazole,ronidazole,dimetridazole and tinidazole were 15.7,25.3,27.3 and 54.9 μg/kg respectively.In the concentration range of 0.1~10.0 μg/ml,the chromatographic peak area of 4 kinds of nitroimidazole drugs showed linear correlation to their concentrations,with correlation coefficients of 0.993~0.999,which was accordant with the demands of antibiotics analysis in feeds.The average recovery of 4 kinds of nitroimidazole drugs were 82.5 %~99.7 %,which was accordant with the accuracy requirement of analyzing drug additives in feeds.Their variation coefficients were 3.5 %~14.0 %,which was accordant with the precision requirement in residue analysis.[Conclusion] The method was simple to operate,with accurate results,which could be used for determining the contents of nitroimidazoles in feeds.更多还原