【摘要】 背景与目的:研究稀土元素镨对蚕豆的遗传毒性和细胞毒性。材料与方法:蚕豆初生幼苗浸入7个PrCl3浓度(1,2,4,8,16,32,64μg/ml)系列溶液,25℃恒温培养6h,之后双蒸水中修复培养24h,切取根尖。进行蚕豆根尖细胞微核试验,计数有丝分裂指数、染色体畸变率。结果:PrCl3浓度在≥8μg/ml时可损伤蚕豆根尖细胞,影响根尖的生长;在1～32μg/ml浓度范围内根尖细胞微核率呈剂量_效应关系(r=0.948,P<0.05);在2～64μg/ml浓度范围内有丝分裂指数呈剂量_效应关系(r=-0.789,P<0.05);在1～8μg/ml浓度范围内染色体畸变率呈剂量_效应关系(r=0.992,P<0.05)。结论:稀土元素镨对蚕豆根尖细胞具有一定的遗传毒性和细胞毒性。更多还原

【Abstract】 BACKGROUND AND AIM:To evaluate the genotoxic and cytotoxic effects of rare earth element praseodymium on the Vicia faba L.root-tip cells.MATERIALS AND METHODS:The primary roots of Vicia faba L.were cultured for 6 hours in various concentrations of PrCl3(64,32,16,8,4,2,1 μg/ml) at 25 ℃,then the roots were restored in double distilled water for 24 h.The root tips of Vicia faba L.were dissected and micronucleus test was performed,mitotic index and frequency of chromosomal aberrations were counted.RESULTS:PrCl3 could cause damage to the root-tip cells of Vicia faba L.attenuated the growth of root-tip with concentration ≥8 μg/ml.Frequency of micronucleus increased in a dose-dependent manner with concentrations of PrCl3 from 1 to 32 μg/ml(r=0.948,P<0.05).Mitosis index rose in a dose-dependent manner when the concentration of PrCl3 increased from 2 to 64 μg/ml(r=-0.789).Frequency of chromosomal aberrations also escalated in a dose-dependent manner when the concentrations of PrCl3 were between 1-8 μg/ml(r=0.992,P<0.05).CONCLUSION:The rare earth element praseodymium has certain genotoxic and cytotoxic effects on the Vicia faba L.root-tip cells.更多还原