【摘要】 背景与目的:探讨丹参酮ⅡA(tanshinoneⅡA,TanⅡA)对人胃癌BGC-823细胞增殖及凋亡的影响。材料与方法:采用0~10μg/ml TanⅡA分别作用于BGC-823细胞48h及72h,MTT比色法观察药物对细胞生长的抑制效应;2、5、10μg/ml TanⅡA分别作用于细胞72h,应用HE染色、DNA琼脂糖凝胶电泳、流式细胞术分析药物对细胞凋亡的影响。结果:TanⅡA明显抑制BGC-823细胞增殖、诱导细胞凋亡,镜下可见BGC-823细胞明显的凋亡特征性改变;5、10μg/ml TanⅡA处理组细胞DNA电泳可见典型的"梯状"条带;FCM结果显示5、10μg/ml TanⅡA处理组细胞,凋亡率分别为(20.60±1.84)%、(31.03±1.47)%,与对照组(5.23±0.39)%比较差异有统计学意义(P<0.01)。结论:在一定条件下,TanⅡA可抑制人胃癌BGC-823细胞增殖,诱导其凋亡。更多还原

【Abstract】 BACKGROUND AND AIM:Effects of tanshinoneⅡA(TanⅡA)on proliferation and apoptosis of the human gastric cancer cell line BGC-823 were studied.MATERIALS AND METHODS:The inhibitory effect on proliferation of BGC-823 cells was evaluated in vitro by using MTT colorimetric assay after cells were treated with TanⅡA at various concentrations(0-10 μg/ml)for 48 h and 72 h.Cell apoptosis was analyzed by HE staining,DNA agarose gel electrophoresis and flow cytometry after BGC-823 cells were treated with 2,5,10 μg/ml TanⅡA for 72 h.RESULTS:TanⅡA inhibited the proliferation of BGC-823 cell markedly.The typical morphological features indicative of apoptotic state were seen under the light microscope.The DNA ladder was generated after treatment with TanⅡA at the concentrations of 5 and 10 μg/ml.The apoptotic rates were(20.60±1.84)% and(31.03±1.47)% in BGC-823 cells treated with 5,10 μg/ml of TanⅡA,respectively.There was marked difference compared with the control group whose apoptotic rate was(5.23±0.39)%.CONCLUSION:TanⅡA could inhibit proliferation and induce apoptosis of human gastric cancer cell line BGC-823 in this test.更多还原