【摘要】 目的:研究大蒜多糖C对免疫性肝损伤小鼠外周血淋巴细胞亚群的影响。方法:采用尾静脉注射卡介苗加脂多糖(BCG+LPS)建立免疫性肝损伤模型,应用流式细胞术测量小鼠外周血淋巴细胞亚群的百分比和CD4+/CD8+比值。结果:模型组T淋巴细胞、CD8+T细胞百分比值较正常对照组明显升高(P<0.01),B淋巴细胞百分比值、CD4+/CD8+比值明显下降(P<0.01),CD4+T细胞百分比也有减少(P<0.05),NK1.1+T细胞百分比无显著变化。大蒜多糖C(800mg/kg ig,qd×12)治疗能使BCG+LPS免疫性肝损伤小鼠外周血T、B、CD4+T和CD8+T淋巴细胞百分比显著下降,而CD4+/CD8+比值显著上升,恢复到正常水平,对NK1.1+T细胞百分含量则无明显影响。结论:大蒜多糖C能全面抑制免疫性肝损伤小鼠的免疫功能,并促使机体建立新的免疫平衡,从而对免疫性肝损伤产生保护作用。更多还原

【Abstract】 Objective: To study the effects of Garlic Polysaccharide C on the peripheral-blood lymphocyte subsets in mice with immunologic liver injury.Method : The immunological liver damage animal model was reproduced by injection via the tail vein of BCG(Bacilli Calmette Guein)and lipopolysaccharide(LPS) into mice.The peripheral-blood lymphocyte subsets′ percentages and the CD4+/CD8+ ratio was measured by the flow cytometry.Results: Compared with normal control group,the percentages of the T lymphocyte and the CD8+ T lymphocyte in the peripheral-blood in immunological liver damage mice were siginificantly increased(P < 0.01).however,the percentages of the B lymphocyte,CD4+ T lymphocyte and the ratio of CD4+/CD8+ decreased(P <0.05 or P < 0.01).Garlic polysaccharide C at dose of 800 mg/kg(ig,qd×12) decreased the percentages of T,B,CD4+ T and CD8+ T lymphocyte in the peripheral-blood in mice treated with BCG+LPS.however,the CD4+/CD8+ ratio increased to the normal level.The percentages of the NK1.1+ T lymphocyte showed no obvious difference between control,BCG+LPS liver injury model and Garlic Polysaccharide C groups.Conclusions: Garlic Polysaccharide C can inhibit the immunologic activity,and establish a new immunologic balance,thereby produce protective effects on immunologic liver injury.更多还原