【摘要】 目的探讨北方地区汉族人急性早幼粒细胞白血病(APL)与人类主要组织相容性复合体MHC—DRB1*基因多态性的关系。方法应用顺序特异性引物和聚合酶链反应(PCR-SSP)技术,对21例急性早幼粒细胞白血病患者及32例无血缘关系的健康人的MHC—DRB1*各等位基因及亚基因进行了检测分析,并将该方法与其它检测MHC等位基因的方法进行对比。结果结果表明,MHC—DRB1*09(RR=1.59,P<0.05=;MHC—DRB1*14(RR=1.59,P<0.05=基因与APL呈正相关,显著高于对照组,两组之间比较差异有显著性意义,而其它MHC—DRB1*各等位基因未见异常,均无统计学差异。结论本项研究结果提示,MHC—DRB1*09和14基因可能是我国北方汉族人APL致病的易感基因,为揭示APL的发病机制中免疫遗传学作用提供了重要信息和依据。更多还原

【Abstract】 Objective: In order to study the correlativity between MHC—DRB1*gene polymorphism and acute promyelocyti leukemia(APL).Methods: MHC—DRB1*gene Polymorphism in 21 patients with APL and 32 normal control subjects was analyzed by using the polymerease chain reaction\sequence specific primer(PCR\SSP)technique.Result: MHC—DRB1*09(RR=1.59,P<0.05=;MHC—DRB1*14(RR=1.59,P<0.05=.The frequency of HLA-A*09 and 14 allele was significantly higher in patients than that in the controls.Conclusion : These findings further Verify that immunogenetics play a predominant Pathogenetics role in a subset of APL patients.Individuals carrying HLA-A*09 and 14 alleles may be susceptibility gene to APL.This method is more specific,sensitive,simple,rapid and accurate,and could be applied to the routine clinical detective.更多还原