【摘要】 目的:观察海珠益肝胶囊对免疫性肝损伤小鼠神经肽SP、VIP及辅助性T细胞亚群的影响。方法:应用尾静脉注射卡介苗(BCG)和脂多糖(LPS)建立免疫性肝损伤小鼠模型,分离小鼠淋巴细胞,经佛波醇乙酯(PMA)、离子霉素、莫能菌素刺激培养6h后,收获细胞,利用细胞内细胞因子染色流式细胞术检测Th1/Th2比例:采用放免法检测血浆和脑垂体中SP、VIP含量。结果:在模型组,外周血和脑垂体中SP含量较正常组逐渐增加,VIP含量较正常组逐渐降低,第18天、25天与正常组比较有显著性差异(P<0.05);Th1细胞的百分率逐渐升高,Th2细胞的百分率逐渐降低,Th1/Th2的比值增大,与肝组织损害程度呈现正相关:海珠益肝胶囊能够降低外周血和脑垂体中SP含量,增加V lP含量,与模型组第25天比较有显著差异(P<0.01),能够恢复SP与V lP的平衡;降低Th1细胞的百分率,恢复Th1/Th2的比例,对肝组织损害具有保护和改善作用(P<0.05)。结论:在小鼠免疫性肝损伤过程中,存在SP与VIP的分泌紊乱及Th1/Th2细胞失衡,海珠益肝胶囊能够恢复SP/VIP、Th1/Th2细胞的平衡,对肝组织损害具有保护利改善作用,可能是其免疫约理机制之一。更多还原

【Abstract】 Objective:To observe the effects of HaiZhu of YiGan Capsule on SP/VIP,Th1/Th2 cells in the peripherl blood mononuclear cells from the mouse with immunological liver injure.Methods:The ratios of Th1/Th2 cells in the mouse with immunological liver injure were detected by flow cytometry with two color intracellular cytokine staining;the changes in substance P(SP),vasoactive intestinal peptide(VIP)in the pituitary gland and peripheral blood were detemined by radio-immunoassary.Results:In the model group,SP contents in the pituitary gland and peripheral blood gradually increased,and VIP contents gradually decreased,and the changes were significant(P<0.05).Th1 cell ratios were higher,Th2 cell ratios were lower,the ratios of Th1/Th2 were higher,there was positive correlative between the ratio of Th1/Th2 and liver injure.HaiZhu of YiGan Capsule could downregulate the levels of SP,and upregulate the levels of VIP,and downregulate the radio of Th1 cell,and could convert the ratio of Th1/Th2,and could improve live pathological injury(P<0.05).Conclusion:There were the disturbance of neuropeptide SP/VIP and Th1/Th2 in the mice with imunological hepatic injury,HaiZhu of YiGan Capsule could convert the imbalance of SP/VIP and Th1/Th2,and make it incline to the normal state,and make it incline to the normal state,and improve liver pathological injury.更多还原